Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3834359..3834900 Replicon chromosome
Accession NZ_CP020835
Organism Escherichia coli strain CFSAN051542

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag BCD20_RS20140 Protein ID WP_000615983.1
Coordinates 3834622..3834900 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag BCD20_RS20135 Protein ID WP_000729703.1
Coordinates 3834359..3834619 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCD20_RS20115 3830034..3830819 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
BCD20_RS20120 3830791..3832503 + 1713 Protein_3690 flagellar biosynthesis protein FlhA -
BCD20_RS20125 3832727..3833224 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BCD20_RS20130 3833400..3834149 - 750 WP_024192679.1 C40 family peptidase -
BCD20_RS20135 3834359..3834619 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BCD20_RS20140 3834622..3834900 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BCD20_RS20145 3835056..3835796 + 741 WP_001225679.1 murein L,D-transpeptidase -
BCD20_RS20150 3835767..3836534 - 768 WP_000333380.1 class II glutamine amidotransferase -
BCD20_RS20155 3836740..3837318 - 579 WP_085381474.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssG / fha / tssJ / tssL / tssM / tssA / hcp1/tssD1 3827200..3858498 31298


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T75485 WP_000615983.1 NZ_CP020835:3834622-3834900 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T75485 NZ_CP020835:3834622-3834900 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT75485 WP_000729703.1 NZ_CP020835:3834359-3834619 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT75485 NZ_CP020835:3834359-3834619 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References