Detailed information of TA system TA075484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3828162..3828856
• Replicon: chromosome
• Accession: NZ_CP020835
• Organism: Escherichia coli strain CFSAN051542

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BCD20_RS20100
• Protein ID: WP_001263493.1
• Coordinates: 3828162..3828560 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BCD20_RS20105
• Protein ID: WP_000554757.1
• Coordinates: 3828563..3828856 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCD20_RS20070	3823162..3824406	-	1245	WP_044864403.1	esterase FrsA	-
-	3823822..3823902	-	81	NuclAT_11	-	-
-	3823822..3823902	-	81	NuclAT_11	-	-
-	3823822..3823902	-	81	NuclAT_11	-	-
-	3823822..3823902	-	81	NuclAT_11	-	-
BCD20_RS20075	3824498..3824956	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BCD20_RS20080	3825217..3826674	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BCD20_RS20085	3826731..3827252	-	522	Protein_3683	peptide chain release factor H	-
BCD20_RS20090	3827251..3827454	-	204	Protein_3684	RNA ligase RtcB family protein	-
BCD20_RS20095	3827700..3828152	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BCD20_RS20100	3828162..3828560	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BCD20_RS20105	3828563..3828856	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BCD20_RS20110	3828908..3829963	-	1056	WP_001226164.1	DNA polymerase IV	-
BCD20_RS20115	3830034..3830819	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
BCD20_RS20120	3830791..3832503	+	1713	Protein_3690	flagellar biosynthesis protein FlhA	-
BCD20_RS20125	3832727..3833224	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssG / fha / tssJ / tssL / tssM / tssA / hcp1/tssD1	3827200..3858498	31298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T75484 WP_001263493.1 NZ_CP020835:c3828560-3828162 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T75484 NZ_CP020835:c3828560-3828162 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT75484 WP_000554757.1 NZ_CP020835:c3828856-3828563 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT75484 NZ_CP020835:c3828856-3828563 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6