Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3575868..3576525 Replicon chromosome
Accession NZ_CP020817
Organism Enterobacter sp. Crenshaw

Toxin (Protein)


Gene name higB Uniprot ID A0A808IF03
Locus tag B7P19_RS17250 Protein ID WP_033146338.1
Coordinates 3575868..3576167 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A808ICP3
Locus tag B7P19_RS17255 Protein ID WP_033146339.1
Coordinates 3576178..3576525 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7P19_RS17240 3572238..3574436 + 2199 WP_048979782.1 type I secretion system permease/ATPase -
B7P19_RS17245 3574447..3575616 + 1170 WP_086374946.1 HlyD family efflux transporter periplasmic adaptor subunit -
B7P19_RS17250 3575868..3576167 + 300 WP_033146338.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B7P19_RS17255 3576178..3576525 + 348 WP_033146339.1 HigA family addiction module antidote protein Antitoxin
B7P19_RS17260 3576570..3576995 + 426 WP_048979783.1 nucleoside triphosphatase NudI -
B7P19_RS17265 3576998..3577507 - 510 WP_048979784.1 hypothetical protein -
B7P19_RS17270 3577592..3578773 - 1182 WP_048979785.1 PLP-dependent aminotransferase family protein -
B7P19_RS17275 3578794..3579681 - 888 WP_048979786.1 LysR family transcriptional regulator -
B7P19_RS17280 3579779..3580381 + 603 WP_048979787.1 short chain dehydrogenase -
B7P19_RS17285 3580375..3581109 - 735 WP_048979788.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11691.32 Da        Isoelectric Point: 10.0346

>T75437 WP_033146338.1 NZ_CP020817:3575868-3576167 [Enterobacter sp. Crenshaw]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T75437 NZ_CP020817:3575868-3576167 [Enterobacter sp. Crenshaw]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13066.02 Da        Isoelectric Point: 7.0134

>AT75437 WP_033146339.1 NZ_CP020817:3576178-3576525 [Enterobacter sp. Crenshaw]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT75437 NZ_CP020817:3576178-3576525 [Enterobacter sp. Crenshaw]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCTCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IF03

Antitoxin

Source ID Structure
AlphaFold DB A0A808ICP3

References