Detailed information of TA system TA075406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3631434..3632039
• Replicon: chromosome
• Accession: NZ_CP020771
• Organism: Microcystis aeruginosa PCC 7806SL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4FMI0
• Locus tag: BH695_RS17960
• Protein ID: WP_002740810.1
• Coordinates: 3631434..3631715 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BH695_RS17965
• Protein ID: WP_036399402.1
• Coordinates: 3631725..3632039 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH695_RS17930	3628811..3629092	+	282	WP_002740820.1	hemolysin XhlA family protein	-
BH695_RS17935	3629204..3629626	-	423	WP_002740818.1	PIN domain-containing protein	-
BH695_RS17940	3629613..3629873	-	261	WP_002740817.1	hypothetical protein	-
BH695_RS17950	3630436..3630642	+	207	WP_002740815.1	type II toxin-antitoxin system HicB family antitoxin	-
BH695_RS17955	3630632..3630853	+	222	WP_036399405.1	type II toxin-antitoxin system HicA family toxin	-
BH695_RS27280	3630979..3631134	+	156	WP_002740811.1	hypothetical protein	-
BH695_RS17960	3631434..3631715	+	282	WP_002740810.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BH695_RS17965	3631725..3632039	+	315	WP_036399402.1	HigA family addiction module antidote protein	Antitoxin
BH695_RS17970	3632208..3635795	+	3588	WP_002740807.1	CHAT domain-containing protein	-
BH695_RS17975	3635943..3636212	+	270	WP_002740805.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11127.76 Da
• Isoelectric Point: 9.7184

>T75406 WP_002740810.1 NZ_CP020771:3631434-3631715 [Microcystis aeruginosa PCC 7806SL]
VIQNFKDKEAQKVFERKHSRKLPLDIQQVALRKLRMLNRAETLQDLRVPPANRLERLVGDREGQYSIRVNDQWRICFVWQ
NGDALDVEIVDYH

Nucleotide

• Length: 282 bp

>T75406 NZ_CP020771:3631434-3631715 [Microcystis aeruginosa PCC 7806SL]
GTGATCCAAAACTTTAAAGATAAAGAAGCTCAAAAAGTTTTTGAACGTAAACATTCGCGTAAATTGCCATTAGATATCCA
ACAAGTGGCATTGCGAAAATTGCGGATGCTTAATCGTGCGGAAACCCTGCAAGATCTTCGTGTCCCACCAGCAAATCGAT
TGGAACGTCTGGTTGGTGATAGGGAAGGTCAATATAGCATTCGGGTTAACGATCAATGGCGAATTTGCTTTGTCTGGCAA
AACGGTGATGCTCTAGACGTAGAAATCGTTGATTATCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11812.70 Da
• Isoelectric Point: 4.0742

>AT75406 WP_036399402.1 NZ_CP020771:3631725-3632039 [Microcystis aeruginosa PCC 7806SL]
MNEDKLMPIHPGEVLLEEFIKPMNLSQNQIALALGVPEQCINEIVHGKQPITADIALRLARYFDMSPRFWLGLQMDYDLD
VVEDEIGDQLDKEVAVMTVICAEK

Nucleotide

• Length: 315 bp

>AT75406 NZ_CP020771:3631725-3632039 [Microcystis aeruginosa PCC 7806SL]
ATGAATGAAGATAAACTAATGCCAATTCATCCCGGTGAGGTTCTTTTAGAGGAATTTATCAAGCCAATGAACCTTAGTCA
GAATCAAATAGCTCTGGCTCTTGGAGTTCCCGAACAATGTATTAACGAAATCGTTCACGGAAAGCAGCCTATCACAGCGG
ATATAGCTCTTCGGTTAGCTCGTTATTTTGATATGTCACCGCGATTTTGGCTGGGATTACAAATGGATTACGATCTGGAC
GTTGTTGAAGATGAAATAGGAGATCAATTGGACAAAGAAGTTGCTGTAATGACAGTTATTTGTGCAGAAAAGTAA

Structures

Toxin

• AlphaFold DB: I4FMI0