75404

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2269313..2269925 Replicon chromosome
Accession NZ_CP020771
Organism Microcystis aeruginosa PCC 7806SL

Toxin (Protein)


Gene name graT Uniprot ID A0A1X9L6L2
Locus tag BH695_RS11250 Protein ID WP_002744781.1
Coordinates 2269644..2269925 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID L7EDF0
Locus tag BH695_RS11245 Protein ID WP_002733868.1
Coordinates 2269313..2269627 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH695_RS11215 2264985..2266304 + 1320 WP_002744798.1 four-carbon acid sugar kinase family protein -
BH695_RS11225 2267017..2267355 + 339 WP_002744788.1 XisI protein -
BH695_RS11230 2267717..2267989 + 273 WP_002744784.1 type II toxin-antitoxin system HicB family antitoxin -
BH695_RS11235 2267967..2268197 + 231 WP_196219943.1 type II toxin-antitoxin system HicA family toxin -
BH695_RS11240 2268285..2268986 + 702 WP_002744782.1 hypothetical protein -
BH695_RS11245 2269313..2269627 - 315 WP_002733868.1 HigA family addiction module antidote protein Antitoxin
BH695_RS11250 2269644..2269925 - 282 WP_002744781.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BH695_RS27100 2269956..2270114 - 159 WP_002744779.1 hypothetical protein -
BH695_RS11255 2270292..2270684 + 393 WP_002744778.1 hypothetical protein -
BH695_RS11260 2270697..2271524 + 828 WP_002744776.1 septum site-determining protein MinC -
BH695_RS11265 2271824..2272624 + 801 WP_002744774.1 septum site-determining protein MinD -
BH695_RS11270 2272654..2272941 + 288 WP_002744772.1 cell division topological specificity factor MinE -
BH695_RS11275 2272938..2273156 + 219 WP_002744770.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11162.76 Da        Isoelectric Point: 10.2927

>T75404 WP_002744781.1 NZ_CP020771:c2269925-2269644 [Microcystis aeruginosa PCC 7806SL]
MIKTFASKETEQVFKRKFSRKLPQDIQRNARRKLEVLDAAETLQDLYIPPSNRLEKLSGDRKNQHSIRINDQWRICFIWK
NGNAYNVEIVDYH

Download         Length: 282 bp

>T75404 NZ_CP020771:c2269925-2269644 [Microcystis aeruginosa PCC 7806SL]
ATGATTAAGACTTTTGCATCTAAGGAGACAGAGCAGGTATTCAAACGTAAATTTTCACGAAAATTACCTCAAGATATTCA
ACGAAATGCTCGGAGAAAGCTAGAAGTTTTAGACGCGGCCGAAACTTTACAGGATTTATACATTCCTCCATCTAATCGGT
TGGAAAAACTCTCAGGAGATAGAAAAAATCAGCATAGTATCCGAATTAACGATCAGTGGCGGATCTGTTTTATTTGGAAA
AATGGCAACGCCTACAATGTAGAAATAGTCGATTATCATTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12332.33 Da        Isoelectric Point: 4.8499

>AT75404 WP_002733868.1 NZ_CP020771:c2269627-2269313 [Microcystis aeruginosa PCC 7806SL]
MTEKKLLAPIHPGEILLEEFLKPMEISQERLAEDLNVPIEQIKEIIEEKRGITADIALRLSRYFGMSERFWMNLQTRYDI
EVEKDRLRERLEKEVKVYAASQAL

Download         Length: 315 bp

>AT75404 NZ_CP020771:c2269627-2269313 [Microcystis aeruginosa PCC 7806SL]
ATGACTGAGAAAAAATTATTAGCCCCCATTCATCCTGGTGAGATTCTGTTAGAAGAATTTCTCAAACCGATGGAAATTAG
CCAAGAACGTTTGGCAGAAGATCTTAATGTACCAATAGAGCAAATCAAGGAAATTATCGAGGAAAAGCGAGGAATTACTG
CTGATATTGCCTTAAGGCTTTCCCGTTATTTTGGTATGTCTGAAAGGTTCTGGATGAATCTTCAAACAAGGTATGATATT
GAAGTAGAAAAAGATAGGCTCAGAGAACGTTTAGAGAAGGAAGTAAAAGTCTATGCTGCTTCACAAGCACTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X9L6L2

Antitoxin

Source ID Structure
AlphaFold DB L7EDF0

References