Detailed information of TA system TA075403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 602100..602923
• Replicon: chromosome
• Accession: NZ_CP020771
• Organism: Microcystis aeruginosa PCC 7806SL

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A1X9L1Q2
• Locus tag: BH695_RS03135
• Protein ID: WP_036399371.1
• Coordinates: 602426..602923 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: BH695_RS03130
• Protein ID: WP_201261447.1
• Coordinates: 602100..602423 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH695_RS25560	598090..598155	+	66	Protein_602	DUF4164 domain-containing protein	-
BH695_RS03110	598193..598879	-	687	WP_084990110.1	N-acetylmannosamine-6-phosphate 2-epimerase	-
BH695_RS03115	599035..599544	-	510	WP_002732961.1	inorganic diphosphatase	-
BH695_RS03125	600640..601818	-	1179	WP_002732401.1	AI-2E family transporter	-
BH695_RS03130	602100..602423	+	324	WP_201261447.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
BH695_RS03135	602426..602923	+	498	WP_036399371.1	type II toxin-antitoxin system YhaV family toxin	Toxin
BH695_RS03140	603277..603492	+	216	Protein_608	IS5/IS1182 family transposase	-
BH695_RS03145	603529..603972	-	444	WP_002740761.1	IS200/IS605 family transposase	-
BH695_RS03150	604013..605316	+	1304	Protein_610	IS5 family transposase	-
BH695_RS03155	605320..605565	+	246	WP_002740773.1	hypothetical protein	-
BH695_RS03160	605640..606710	+	1071	Protein_612	IS630 family transposase	-
BH695_RS03165	606795..607820	-	1026	WP_002732998.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (14-151)

Antitoxin

• PrlF antitoxin (6-104)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 19635.32 Da
• Isoelectric Point: 8.3819

>T75403 WP_036399371.1 NZ_CP020771:602426-602923 [Microcystis aeruginosa PCC 7806SL]
MSIDQPLVINGWSIFAHPLFLEQFEELVTQVEELRHKFPQDYKRKNATKLLAAIAKLAFDTIPEDPTRSEYRQGTALGDD
YKHWFRAKFFQQYRLFFRYHQESKIILLAWVNDQNSKRAFESSTDAYRVFRKMLETGYPPDDWHDLLKEAKSTISRLRKA
AETEI

Nucleotide

• Length: 498 bp

>T75403 NZ_CP020771:602426-602923 [Microcystis aeruginosa PCC 7806SL]
TTGTCTATAGATCAGCCCCTAGTAATTAATGGATGGAGCATATTTGCTCATCCTCTGTTCCTTGAACAGTTTGAAGAACT
TGTGACGCAGGTTGAAGAGCTGCGCCACAAGTTTCCTCAAGATTACAAAAGGAAAAATGCGACAAAGCTTCTAGCTGCCA
TAGCAAAGTTAGCCTTTGATACTATTCCTGAAGACCCAACTCGCAGTGAGTATCGACAGGGAACTGCGCTTGGAGACGAT
TATAAACACTGGTTTAGAGCCAAGTTTTTTCAGCAGTATCGACTGTTTTTTCGATATCATCAAGAGAGTAAAATAATTCT
CCTCGCTTGGGTCAATGATCAAAATTCTAAGCGAGCTTTTGAGAGTAGTACAGACGCTTATAGAGTCTTTAGAAAAATGC
TGGAGACCGGTTATCCTCCAGATGATTGGCATGACCTACTCAAAGAGGCCAAAAGTACAATTAGCCGTTTACGCAAAGCT
GCTGAAACCGAAATTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12120.57 Da
• Isoelectric Point: 4.2675

>AT75403 WP_201261447.1 NZ_CP020771:602100-602423 [Microcystis aeruginosa PCC 7806SL]
MAPWPESTLTDRYQTTIPDSVRKALGLNKRDKICYNIQPDGKVWISRAEGTEENNPVLGKFLNFLAQDMEQNPQHIQPIS
SNLVNHVQYLVSGVDLDIDAPLLDEDE

Nucleotide

• Length: 324 bp

>AT75403 NZ_CP020771:602100-602423 [Microcystis aeruginosa PCC 7806SL]
ATGGCTCCATGGCCAGAATCCACTCTTACCGATCGCTATCAGACTACAATTCCCGATTCCGTTCGCAAAGCCCTTGGCTT
GAATAAGCGCGATAAAATCTGCTATAACATACAGCCCGACGGCAAAGTATGGATTTCTAGGGCTGAGGGGACAGAAGAAA
ATAATCCGGTGCTTGGGAAATTTCTGAATTTTCTTGCCCAAGATATGGAACAGAACCCTCAACACATACAGCCAATTAGT
TCTAACCTAGTCAATCATGTTCAATACTTGGTCTCTGGTGTTGATCTGGATATTGATGCACCACTTCTGGATGAGGACGA
ATAA

Structures

Toxin

• AlphaFold DB: A0A1X9L1Q2