Detailed information of TA system TA075385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1396007..1396232
• Replicon: chromosome
• Accession: NZ_CP020753
• Organism: Shigella flexneri 1c strain Y394

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: B7485_RS07195
• Protein ID: WP_000813254.1
• Coordinates: 1396077..1396232 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1396007..1396065 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7485_RS25515	1392005..1392174	+	170	Protein_1385	hypothetical protein	-
B7485_RS07160	1392320..1393066	+	747	WP_000788996.1	ATP-binding protein	-
B7485_RS07165	1393081..1393503	+	423	WP_001118167.1	DUF977 family protein	-
B7485_RS07170	1393561..1393917	+	357	WP_005048249.1	hypothetical protein	-
B7485_RS07175	1394010..1394228	+	219	WP_000256998.1	DUF4014 family protein	-
B7485_RS07180	1394230..1394595	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
B7485_RS07185	1394592..1395257	+	666	WP_000208062.1	hypothetical protein	-
B7485_RS07190	1395257..1395622	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1396007..1396065	-	59	-	-	Antitoxin
B7485_RS07195	1396077..1396232	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
B7485_RS07210	1397569..1398168	+	600	WP_000940329.1	DUF1367 family protein	-
B7485_RS07215	1398168..1398458	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
B7485_RS07220	1398455..1399009	+	555	WP_000640143.1	DUF1133 family protein	-
B7485_RS07225	1399150..1399335	+	186	WP_005049799.1	hypothetical protein	-
B7485_RS07230	1399397..1400553	+	1157	WP_134797302.1	IS3-like element IS600 family transposase	-
B7485_RS07235	1400546..1401106	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1387780..1433473	45693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T75385 WP_000813254.1 NZ_CP020753:1396077-1396232 [Shigella flexneri 1c]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T75385 NZ_CP020753:1396077-1396232 [Shigella flexneri 1c]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT75385 NZ_CP020753:c1396065-1396007 [Shigella flexneri 1c]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin