Detailed information of TA system TA075383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 750547..751075
• Replicon: chromosome
• Accession: NZ_CP020753
• Organism: Shigella flexneri 1c strain Y394

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B7485_RS03805
• Protein ID: WP_005061113.1
• Coordinates: 750845..751075 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: B7485_RS03800
• Protein ID: WP_000147081.1
• Coordinates: 750547..750855 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7485_RS03775 (747312)	747312..748480	+	1169	WP_000747032.1	IS3-like element IS911 family transposase	-
B7485_RS03780 (748805)	748805..749572	+	768	Protein_727	hypothetical protein	-
B7485_RS03785 (749647)	749647..749925	+	279	WP_005061104.1	hypothetical protein	-
B7485_RS03790 (749922)	749922..750332	+	411	WP_000814621.1	recombination protein NinB	-
B7485_RS03795 (750329)	750329..750505	+	177	WP_024260406.1	NinE family protein	-
B7485_RS03800 (750547)	750547..750855	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
B7485_RS03805 (750845)	750845..751075	-	231	WP_005061113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7485_RS03810 (751088)	751088..751546	+	459	WP_000961311.1	ash family protein	-
B7485_RS03815 (751604)	751604..752056	+	453	WP_232046050.1	Rha family transcriptional regulator	-
B7485_RS03820 (752053)	752053..752286	+	234	WP_000618004.1	hypothetical protein	-
B7485_RS03825 (752273)	752273..753181	+	909	WP_001247840.1	hypothetical protein	-
B7485_RS03830 (753192)	753192..754070	+	879	WP_000988183.1	ATP-binding protein	-
B7485_RS03835 (754067)	754067..755458	+	1392	WP_000211324.1	replicative DNA helicase	-
B7485_RS03840 (755455)	755455..755712	+	258	WP_001064799.1	hypothetical protein	-
B7485_RS03845 (755807)	755807..756007	+	201	WP_000111769.1	protein ninH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	691821..787933	96112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8665.12 Da
• Isoelectric Point: 10.5308

>T75383 WP_005061113.1 NZ_CP020753:c751075-750845 [Shigella flexneri 1c]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T75383 NZ_CP020753:c751075-750845 [Shigella flexneri 1c]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT75383 WP_000147081.1 NZ_CP020753:c750855-750547 [Shigella flexneri 1c]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT75383 NZ_CP020753:c750855-750547 [Shigella flexneri 1c]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1