Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 413201..413819 Replicon chromosome
Accession NZ_CP020753
Organism Shigella flexneri 1c strain Y394

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B7485_RS02020 Protein ID WP_001291435.1
Coordinates 413201..413419 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag B7485_RS02025 Protein ID WP_000344797.1
Coordinates 413445..413819 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7485_RS01990 (409093) 409093..409404 - 312 WP_000409911.1 MGMT family protein -
B7485_RS02000 (409783) 409783..410136 + 354 WP_000878140.1 DUF1428 domain-containing protein -
B7485_RS02005 (410178) 410178..411728 - 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B7485_RS02010 (411892) 411892..412362 - 471 WP_000136192.1 YlaC family protein -
B7485_RS02015 (412478) 412478..413029 - 552 WP_000102577.1 maltose O-acetyltransferase -
B7485_RS02020 (413201) 413201..413419 - 219 WP_001291435.1 HHA domain-containing protein Toxin
B7485_RS02025 (413445) 413445..413819 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
B7485_RS02030 (414365) 414365..417514 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
B7485_RS02035 (417537) 417537..418730 - 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 407735..409063 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T75382 WP_001291435.1 NZ_CP020753:c413419-413201 [Shigella flexneri 1c]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T75382 NZ_CP020753:c413419-413201 [Shigella flexneri 1c]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT75382 WP_000344797.1 NZ_CP020753:c413819-413445 [Shigella flexneri 1c]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT75382 NZ_CP020753:c413819-413445 [Shigella flexneri 1c]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References