Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 300100..300794 Replicon chromosome
Accession NZ_CP020753
Organism Shigella flexneri 1c strain Y394

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag B7485_RS01420 Protein ID WP_001263491.1
Coordinates 300396..300794 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag B7485_RS01415 Protein ID WP_000554759.1
Coordinates 300100..300393 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7485_RS01395 (295739) 295739..296236 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
B7485_RS01400 (296453) 296453..298165 - 1713 Protein_280 flagellar biosynthesis protein FlhA -
B7485_RS01405 (298137) 298137..298922 + 786 WP_000207561.1 putative lateral flagellar export/assembly protein LafU -
B7485_RS01410 (298993) 298993..300048 + 1056 WP_128882050.1 DNA polymerase IV -
B7485_RS01415 (300100) 300100..300393 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
B7485_RS01420 (300396) 300396..300794 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
B7485_RS01425 (300804) 300804..301256 + 453 WP_001059861.1 GNAT family N-acetyltransferase -
B7485_RS01430 (301502) 301502..302545 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
B7485_RS01435 (302607) 302607..303221 + 615 Protein_287 peptide chain release factor H -
B7485_RS01440 (303278) 303278..304735 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
B7485_RS01445 (304997) 304997..305455 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 293180..343039 49859


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T75380 WP_001263491.1 NZ_CP020753:300396-300794 [Shigella flexneri 1c]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T75380 NZ_CP020753:300396-300794 [Shigella flexneri 1c]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT75380 WP_000554759.1 NZ_CP020753:300100-300393 [Shigella flexneri 1c]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT75380 NZ_CP020753:300100-300393 [Shigella flexneri 1c]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References