Detailed information of TA system TA075336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2509151..2509674
• Replicon: chromosome
• Accession: NZ_CP020714
• Organism: Staphylococcus aureus strain K18

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YCD0
• Locus tag: B7474_RS13120
• Protein ID: WP_000074043.1
• Coordinates: 2509408..2509674 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YBB2
• Locus tag: B7474_RS13115
• Protein ID: WP_000587618.1
• Coordinates: 2509151..2509408 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7474_RS13090	2504226..2505419	+	1194	WP_000675407.1	MFS transporter	-
B7474_RS13095	2505650..2506396	-	747	WP_101766504.1	hypothetical protein	-
B7474_RS13100	2506591..2508168	+	1578	WP_101766505.1	FMN-binding glutamate synthase family protein	-
B7474_RS13105	2508336..2508791	-	456	WP_000732076.1	DUF1307 domain-containing protein	-
B7474_RS13115	2509151..2509408	+	258	WP_000587618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B7474_RS13120	2509408..2509674	+	267	WP_000074043.1	Txe/YoeB family addiction module toxin	Toxin
B7474_RS13125	2510030..2511106	+	1077	WP_001076673.1	M42 family metallopeptidase	-
B7474_RS13130	2511354..2512016	+	663	WP_000923524.1	ATP-binding cassette domain-containing protein	-
B7474_RS13135	2512009..2512785	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
B7474_RS13140	2513181..2514368	+	1188	WP_000026194.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10657.45 Da
• Isoelectric Point: 10.3584

>T75336 WP_000074043.1 NZ_CP020714:2509408-2509674 [Staphylococcus aureus]
MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQSFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T75336 NZ_CP020714:2509408-2509674 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9762.78 Da
• Isoelectric Point: 4.1958

>AT75336 WP_000587618.1 NZ_CP020714:2509151-2509408 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT75336 NZ_CP020714:2509151-2509408 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: T1YCD0

Antitoxin

• AlphaFold DB: T1YBB2