Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5270875..5271556 Replicon chromosome
Accession NZ_CP020704
Organism Pseudomonas aeruginosa strain PASGNDM699

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag B7W87_RS25645 Protein ID WP_015503432.1
Coordinates 5271191..5271556 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag B7W87_RS25640 Protein ID WP_004364117.1
Coordinates 5270875..5271198 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7W87_RS25610 5266651..5267228 + 578 Protein_4939 plasmid pRiA4b ORF-3 family protein -
B7W87_RS25625 5269249..5269803 - 555 WP_074197746.1 tyrosine-type recombinase/integrase -
B7W87_RS25630 5269978..5270253 - 276 WP_004364115.1 hypothetical protein -
B7W87_RS25635 5270253..5270585 - 333 WP_016561578.1 hypothetical protein -
B7W87_RS33975 5270640..5270759 + 120 Protein_4944 IS5/IS1182 family transposase -
B7W87_RS25640 5270875..5271198 - 324 WP_004364117.1 XRE family transcriptional regulator Antitoxin
B7W87_RS25645 5271191..5271556 - 366 WP_015503432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B7W87_RS25650 5271820..5272059 - 240 WP_044069615.1 hypothetical protein -
B7W87_RS25655 5272266..5272538 + 273 WP_004364121.1 hypothetical protein -
B7W87_RS25660 5272569..5272994 - 426 WP_003116492.1 VOC family protein -
B7W87_RS25665 5273095..5273979 + 885 WP_004364122.1 LysR family transcriptional regulator -
B7W87_RS25670 5273952..5274905 - 954 WP_003085661.1 LysR family transcriptional regulator -
B7W87_RS25675 5275126..5275560 + 435 WP_003158601.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5237432..5272994 35562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-86)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13798.14 Da        Isoelectric Point: 4.8219

>T75311 WP_015503432.1 NZ_CP020704:c5271556-5271191 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Download         Length: 366 bp

>T75311 NZ_CP020704:c5271556-5271191 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11896.73 Da        Isoelectric Point: 6.4751

>AT75311 WP_004364117.1 NZ_CP020704:c5271198-5270875 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT75311 NZ_CP020704:c5271198-5270875 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References