Detailed information of TA system TA075303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5818353..5818948
• Replicon: chromosome
• Accession: NZ_CP020703
• Organism: Pseudomonas aeruginosa strain PASGNDM345

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: B7W86_RS28095
• Protein ID: WP_003113526.1
• Coordinates: 5818670..5818948 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7W86_RS28090
• Protein ID: WP_003133769.1
• Coordinates: 5818353..5818658 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7W86_RS28060	5813375..5813665	-	291	WP_031757220.1	DUF5447 family protein	-
B7W86_RS28065	5813841..5814113	-	273	WP_004352675.1	hypothetical protein	-
B7W86_RS28075	5814553..5814888	+	336	WP_023094905.1	hypothetical protein	-
B7W86_RS33445	5814982..5815572	+	591	WP_071536478.1	SLATT domain-containing protein	-
B7W86_RS28080	5815569..5816834	+	1266	WP_023094906.1	nucleotidyltransferase	-
B7W86_RS28085	5816918..5817910	-	993	WP_003133766.1	hypothetical protein	-
B7W86_RS28090	5818353..5818658	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
B7W86_RS28095	5818670..5818948	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7W86_RS28105	5819277..5821505	+	2229	WP_003133778.1	TonB-dependent receptor	-
B7W86_RS28110	5821576..5822223	-	648	WP_003095021.1	carbonate dehydratase	-
B7W86_RS28115	5822285..5823523	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T75303 WP_003113526.1 NZ_CP020703:c5818948-5818670 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T75303 NZ_CP020703:c5818948-5818670 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT75303 WP_003133769.1 NZ_CP020703:c5818658-5818353 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT75303 NZ_CP020703:c5818658-5818353 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3