Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4554453..4555059 Replicon chromosome
Accession NZ_CP020664
Organism Microcystis sp. MC19

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag B5D77_RS23040 Protein ID WP_106909717.1
Coordinates 4554453..4554734 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag B5D77_RS23045 Protein ID WP_002801551.1
Coordinates 4554745..4555059 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5D77_RS23030 4549938..4551185 - 1248 WP_106909716.1 tryptophan synthase subunit beta -
B5D77_RS23035 4551323..4553893 - 2571 WP_106910056.1 DUF3536 domain-containing protein -
B5D77_RS23040 4554453..4554734 + 282 WP_106909717.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B5D77_RS23045 4554745..4555059 + 315 WP_002801551.1 HigA family addiction module antidote protein Antitoxin
B5D77_RS23050 4555246..4555506 - 261 WP_002735847.1 Txe/YoeB family addiction module toxin -
B5D77_RS23055 4555487..4555741 - 255 WP_008199246.1 hypothetical protein -
B5D77_RS26385 4555832..4556011 + 180 WP_173915176.1 hypothetical protein -
B5D77_RS23060 4556150..4557457 + 1308 WP_106909718.1 PEP-CTERM sorting domain-containing protein -
B5D77_RS23065 4557840..4558181 - 342 WP_002801547.1 type II toxin-antitoxin system PemK/MazF family toxin -
B5D77_RS23070 4558178..4558411 - 234 WP_008199250.1 hypothetical protein -
B5D77_RS23080 4558612..4558833 - 222 WP_002801544.1 hypothetical protein -
B5D77_RS23085 4558799..4559071 - 273 WP_002777949.1 BrnT family toxin -
B5D77_RS23090 4559189..4559473 - 285 WP_106909720.1 type II toxin-antitoxin system YafQ family toxin -
B5D77_RS23095 4559466..4559684 - 219 WP_002757428.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(25-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(7-96)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10888.36 Da        Isoelectric Point: 8.5233

>T75287 WP_106909717.1 NZ_CP020664:4554453-4554734 [Microcystis sp. MC19]
MIQSFRCKHTRLLFEGGDPPRFRGFKDTAIRKLTQLDAAQTLEFLRSPPGNQLEALKDDRKEQYSIRINSQFRLCFIWTN
NGPTDVEIVDYHS

Download         Length: 282 bp

>T75287 NZ_CP020664:4554453-4554734 [Microcystis sp. MC19]
ATGATTCAATCTTTCCGATGCAAGCATACGCGATTACTTTTTGAAGGAGGAGATCCGCCTCGTTTTCGTGGTTTCAAAGA
TACTGCTATTCGGAAGTTGACTCAATTAGATGCGGCTCAAACCTTAGAGTTTCTGCGCTCTCCACCAGGCAATCAACTTG
AAGCTTTAAAAGACGATAGAAAAGAACAATATAGTATTCGGATTAATTCTCAGTTTCGATTATGTTTTATCTGGACAAAT
AATGGACCTACTGATGTTGAAATTGTTGATTATCACTCATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11736.60 Da        Isoelectric Point: 8.0407

>AT75287 WP_002801551.1 NZ_CP020664:4554745-4555059 [Microcystis sp. MC19]
MNNRPINQMRPVHPGEILREDFLIPLNMSVNALALALKVPASRIHEIVKERRSISADTAARLACYFGGDAASWLSLQVIY
DLKTLSNREEIIQQIQPRISEQIA

Download         Length: 315 bp

>AT75287 NZ_CP020664:4554745-4555059 [Microcystis sp. MC19]
ATGAATAACCGTCCTATTAATCAAATGCGACCCGTTCACCCAGGAGAAATTCTGCGGGAAGATTTTTTGATTCCTCTAAA
TATGAGCGTGAATGCTTTAGCTTTAGCCTTGAAGGTTCCTGCATCTCGTATTCATGAAATTGTTAAAGAACGTCGTTCTA
TTTCGGCTGATACGGCAGCAAGATTAGCCTGCTATTTTGGCGGAGATGCTGCTTCCTGGCTCAGTCTTCAAGTAATTTAT
GATCTGAAGACTTTATCTAACCGTGAGGAGATTATTCAGCAAATTCAGCCCCGTATTTCCGAACAAATTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References