Detailed information of TA system TA075286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3275798..3276403
• Replicon: chromosome
• Accession: NZ_CP020664
• Organism: Microcystis sp. MC19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4FMI0
• Locus tag: B5D77_RS16695
• Protein ID: WP_002740810.1
• Coordinates: 3276122..3276403 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B5D77_RS16690
• Protein ID: WP_002737909.1
• Coordinates: 3275798..3276112 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5D77_RS16675	3275028..3275249	-	222	WP_002800940.1	type II toxin-antitoxin system HicA family toxin	-
B5D77_RS16680	3275239..3275445	-	207	WP_002740815.1	type II toxin-antitoxin system HicB family antitoxin	-
B5D77_RS16690	3275798..3276112	-	315	WP_002737909.1	HigA family addiction module antidote protein	Antitoxin
B5D77_RS16695	3276122..3276403	-	282	WP_002740810.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5D77_RS16700	3276892..3277092	-	201	WP_106909232.1	type II toxin-antitoxin system HicA family toxin	-
B5D77_RS16705	3277085..3277291	-	207	WP_039900529.1	type II toxin-antitoxin system HicB family antitoxin	-
B5D77_RS16720	3277738..3278181	+	444	Protein_3105	DUF29 domain-containing protein	-
B5D77_RS16725	3278231..3279481	-	1251	WP_106909233.1	nickel pincer cofactor biosynthesis protein LarC	-
B5D77_RS16730	3279458..3280099	+	642	WP_008206028.1	threonylcarbamoyl-AMP synthase	-
B5D77_RS16740	3280472..3280882	+	411	WP_008206026.1	Hsp20/alpha crystallin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-92)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11127.76 Da
• Isoelectric Point: 9.7184

>T75286 WP_002740810.1 NZ_CP020664:c3276403-3276122 [Microcystis sp. MC19]
VIQNFKDKEAQKVFERKHSRKLPLDIQQVALRKLRMLNRAETLQDLRVPPANRLERLVGDREGQYSIRVNDQWRICFVWQ
NGDALDVEIVDYH

Nucleotide

• Length: 282 bp

>T75286 NZ_CP020664:c3276403-3276122 [Microcystis sp. MC19]
GTGATCCAAAACTTTAAAGATAAAGAAGCTCAAAAAGTTTTTGAACGTAAACATTCGCGTAAATTGCCATTAGATATCCA
ACAAGTGGCATTGCGAAAATTGCGGATGCTTAATCGTGCGGAAACCCTGCAAGATCTTCGTGTCCCACCAGCAAATCGAT
TGGAACGTCTGGTTGGTGACAGGGAAGGTCAATATAGCATTCGGGTTAACGATCAATGGCGAATTTGCTTTGTCTGGCAA
AACGGTGATGCTCTAGACGTAGAAATCGTTGATTATCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11846.78 Da
• Isoelectric Point: 4.1648

>AT75286 WP_002737909.1 NZ_CP020664:c3276112-3275798 [Microcystis sp. MC19]
MNEDKLMPIHPGEVLLEEFIKPMNLSQNQIALALGVPEQCINEIVHGKRCITADIALRLARYFDMSPRFWLGLQMDYDLD
VVEDEIGDQLDKEVAVMTVICAEK

Nucleotide

• Length: 315 bp

>AT75286 NZ_CP020664:c3276112-3275798 [Microcystis sp. MC19]
ATGAATGAAGATAAACTAATGCCAATTCATCCCGGTGAGGTTCTTTTAGAGGAATTTATCAAGCCAATGAACCTTAGTCA
GAATCAAATAGCTCTGGCTCTTGGAGTTCCTGAACAATGTATTAACGAAATCGTTCACGGAAAGCGGTGTATCACGGCGG
ATATAGCTCTTCGGTTAGCTCGTTATTTTGATATGTCACCGCGATTTTGGCTGGGATTACAAATGGATTACGATCTGGAC
GTTGTTGAAGATGAAATAGGAGATCAATTGGACAAAGAAGTTGCTGTAATGACAGTTATTTGTGCAGAAAAGTAA

Structures

Toxin

• AlphaFold DB: I4FMI0