Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2149684..2150296 Replicon chromosome
Accession NZ_CP020664
Organism Microcystis sp. MC19

Toxin (Protein)


Gene name graT Uniprot ID A0A1V4BLH3
Locus tag B5D77_RS11000 Protein ID WP_052277943.1
Coordinates 2149684..2149965 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID L7EDF0
Locus tag B5D77_RS11005 Protein ID WP_002733868.1
Coordinates 2149982..2150296 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5D77_RS10960 2145137..2145964 - 828 WP_106908821.1 septum site-determining protein MinC -
B5D77_RS10965 2145977..2146327 - 351 WP_079210267.1 hypothetical protein -
B5D77_RS10970 2146536..2147750 - 1215 WP_106908822.1 transposase -
B5D77_RS10980 2147853..2148362 + 510 WP_106908823.1 transposase -
B5D77_RS10985 2148359..2148684 + 326 Protein_2037 sterol desaturase -
B5D77_RS10990 2148784..2149104 + 321 WP_002777378.1 hypothetical protein -
B5D77_RS10995 2149094..2149537 + 444 WP_106908824.1 type II toxin-antitoxin system YhaV family toxin -
B5D77_RS11000 2149684..2149965 + 282 WP_052277943.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B5D77_RS11005 2149982..2150296 + 315 WP_002733868.1 HigA family addiction module antidote protein Antitoxin
B5D77_RS11010 2150330..2150671 - 342 WP_106908825.1 hypothetical protein -
B5D77_RS11015 2150838..2152157 - 1320 WP_106908826.1 four-carbon acid sugar kinase family protein -
B5D77_RS11020 2152307..2153143 + 837 WP_106908827.1 acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase -
B5D77_RS11025 2153337..2154542 + 1206 WP_106909990.1 lipid-A-disaccharide synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2146536..2147750 1214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11162.75 Da        Isoelectric Point: 10.2927

>T75283 WP_052277943.1 NZ_CP020664:2149684-2149965 [Microcystis sp. MC19]
MIKSFASKETEQLFKRKFSRKLPQDIQRNARRKLEVLDAAETLQDLYIPPSNRLEKLSGDRKNQHSIRINDQWRICFIWK
NGNAYNVEIVDYH

Download         Length: 282 bp

>T75283 NZ_CP020664:2149684-2149965 [Microcystis sp. MC19]
ATGATTAAGAGTTTTGCATCTAAGGAGACAGAGCAGTTATTCAAACGTAAATTTTCACGAAAATTACCTCAAGATATTCA
ACGAAATGCTCGGAGAAAGCTAGAAGTTTTAGACGCGGCCGAAACTTTACAGGATTTATACATTCCTCCATCTAATCGGT
TGGAAAAACTCTCAGGAGATAGAAAAAATCAGCATAGTATCCGAATTAACGATCAGTGGCGGATCTGTTTTATTTGGAAA
AATGGCAACGCCTACAATGTAGAAATAGTCGATTATCATTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12332.33 Da        Isoelectric Point: 4.8499

>AT75283 WP_002733868.1 NZ_CP020664:2149982-2150296 [Microcystis sp. MC19]
MTEKKLLAPIHPGEILLEEFLKPMEISQERLAEDLNVPIEQIKEIIEEKRGITADIALRLSRYFGMSERFWMNLQTRYDI
EVEKDRLRERLEKEVKVYAASQAL

Download         Length: 315 bp

>AT75283 NZ_CP020664:2149982-2150296 [Microcystis sp. MC19]
ATGACTGAGAAAAAATTATTAGCCCCCATTCATCCTGGTGAGATTCTGTTGGAAGAATTTCTCAAACCGATGGAAATTAG
CCAAGAACGTTTGGCAGAAGATCTTAATGTACCAATAGAGCAAATCAAGGAAATTATCGAGGAAAAGCGAGGAATTACTG
CCGATATTGCCTTAAGGCTTTCTCGTTATTTTGGTATGTCTGAAAGGTTCTGGATGAATCTTCAAACAAGGTATGATATT
GAAGTAGAAAAAGATAGGCTCAGAGAACGTTTAGAGAAGGAAGTAAAAGTCTATGCTGCTTCACAAGCACTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V4BLH3

Antitoxin

Source ID Structure
AlphaFold DB L7EDF0

References