Detailed information of TA system TA075277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4563295..4563890
• Replicon: chromosome
• Accession: NZ_CP020659
• Organism: Pseudomonas aeruginosa PAK

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: Y880_RS21835
• Protein ID: WP_003113526.1
• Coordinates: 4563612..4563890 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y880_RS21830
• Protein ID: WP_003113527.1
• Coordinates: 4563295..4563600 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y880_RS21800	4558965..4559255	-	291	WP_016253916.1	DUF5447 family protein	-
Y880_RS21810	4559467..4559739	-	273	WP_003115921.1	hypothetical protein	-
Y880_RS21815	4559849..4560115	+	267	WP_016253917.1	hypothetical protein	-
Y880_RS21820	4560171..4560506	+	336	WP_031759015.1	hypothetical protein	-
Y880_RS21825	4560541..4561686	+	1146	WP_016253918.1	DUF262 domain-containing protein	-
Y880_RS30160	4561689..4562456	+	768	WP_016253919.1	hypothetical protein	-
Y880_RS21830	4563295..4563600	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
Y880_RS21835	4563612..4563890	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y880_RS21845	4564219..4566447	+	2229	WP_016253920.1	TonB-dependent receptor	-
Y880_RS21850	4566517..4567161	-	645	WP_016253921.1	carbonate dehydratase	-
Y880_RS21855	4567223..4568461	-	1239	WP_016253922.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T75277 WP_003113526.1 NZ_CP020659:c4563890-4563612 [Pseudomonas aeruginosa PAK]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T75277 NZ_CP020659:c4563890-4563612 [Pseudomonas aeruginosa PAK]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT75277 WP_003113527.1 NZ_CP020659:c4563600-4563295 [Pseudomonas aeruginosa PAK]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT75277 NZ_CP020659:c4563600-4563295 [Pseudomonas aeruginosa PAK]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3