Detailed information of TA system TA075271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3853441..3854101
• Replicon: chromosome
• Accession: NZ_CP020657
• Organism: Klebsiella sp. M5al

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A285B6D8
• Locus tag: BWI76_RS18580
• Protein ID: WP_004132737.1
• Coordinates: 3853748..3854101 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E1DQ94
• Locus tag: BWI76_RS18575
• Protein ID: WP_004132739.1
• Coordinates: 3853441..3853743 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI76_RS18545	3848447..3849901	+	1455	WP_049089250.1	AMP nucleosidase	-
BWI76_RS18555	3850929..3852386	-	1458	WP_004136541.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
BWI76_RS18565	3852710..3853043	-	334	Protein_3559	type II toxin-antitoxin system PemK/MazF family toxin	-
BWI76_RS18570	3853044..3853247	-	204	Protein_3560	hypothetical protein	-
BWI76_RS18575	3853441..3853743	-	303	WP_004132739.1	XRE family transcriptional regulator	Antitoxin
BWI76_RS18580	3853748..3854101	-	354	WP_004132737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWI76_RS18585	3854485..3855903	-	1419	WP_004132735.1	heavy metal sensor histidine kinase	-
BWI76_RS18590	3855903..3856571	-	669	WP_004132733.1	heavy metal response regulator transcription factor	-
BWI76_RS18595	3856711..3857337	+	627	WP_004132731.1	hypothetical protein	-
BWI76_RS18600	3857352..3857960	+	609	WP_004132729.1	cytochrome b/b6 domain-containing protein	-
BWI76_RS18605	3857950..3858717	+	768	WP_004132727.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13554.49 Da
• Isoelectric Point: 10.0045

>T75271 WP_004132737.1 NZ_CP020657:c3854101-3853748 [Klebsiella sp. M5al]
VWMIKTTDTFERWFTSFNDTDRASVLAALLVLREKGPGLSRPNADTLRGSRYSNMKELRIQSRGEPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPVAEREFTNWLKTFKEKE

Nucleotide

• Length: 354 bp

>T75271 NZ_CP020657:c3854101-3853748 [Klebsiella sp. M5al]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGTTCAACGATACCGATAGGGCCAGCGTGCTTGC
AGCGTTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTAATGCCGATACGCTGAGAGGCTCCCGCTACAGCA
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGTAATAAGGTTGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGAGCGGGAATTCAC
GAACTGGCTGAAGACATTCAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11072.83 Da
• Isoelectric Point: 5.7424

>AT75271 WP_004132739.1 NZ_CP020657:c3853743-3853441 [Klebsiella sp. M5al]
MGRTLEQLMADEKPEVVADARAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFEL

Nucleotide

• Length: 303 bp

>AT75271 NZ_CP020657:c3853743-3853441 [Klebsiella sp. M5al]
ATGGGCAGAACGTTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAGATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCGGATGGTTCTCACTACGGATTTGAACTGTAA

Structures

Toxin

• AlphaFold DB: A0A285B6D8