Detailed information of TA system TA075228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2623245..2623858
• Replicon: chromosome
• Accession: NZ_CP020651
• Organism: Bordetella bronchiseptica strain D755

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: B7P00_RS12310
• Protein ID: WP_003811545.1
• Coordinates: 2623245..2623556 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7P00_RS12315
• Protein ID: WP_003811547.1
• Coordinates: 2623559..2623858 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7P00_RS12285	2618363..2618950	-	588	WP_003811534.1	LamB/YcsF family protein	-
B7P00_RS12290	2618947..2620341	-	1395	WP_003811536.1	arylsulfatase	-
B7P00_RS12295	2620356..2621318	-	963	WP_003811538.1	tripartite tricarboxylate transporter substrate binding protein	-
B7P00_RS12300	2621436..2622389	+	954	WP_033451213.1	LysR family transcriptional regulator	-
B7P00_RS12305	2622508..2623131	+	624	WP_003811542.1	glutathione S-transferase family protein	-
B7P00_RS12310	2623245..2623556	+	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7P00_RS12315	2623559..2623858	+	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
B7P00_RS12320	2623872..2626946	-	3075	WP_033455244.1	autotransporter SphB2	-
B7P00_RS12325	2627076..2627750	+	675	WP_003811550.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T75228 WP_003811545.1 NZ_CP020651:2623245-2623556 [Bordetella bronchiseptica]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T75228 NZ_CP020651:2623245-2623556 [Bordetella bronchiseptica]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT75228 WP_003811547.1 NZ_CP020651:2623559-2623858 [Bordetella bronchiseptica]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT75228 NZ_CP020651:2623559-2623858 [Bordetella bronchiseptica]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3