Detailed information of TA system TA075214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1728005..1728618
• Replicon: chromosome
• Accession: NZ_CP020648
• Organism: Bordetella parapertussis strain E738

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: B7O99_RS08075
• Protein ID: WP_003811545.1
• Coordinates: 1728307..1728618 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7O99_RS08070
• Protein ID: WP_003811547.1
• Coordinates: 1728005..1728304 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7O99_RS08060	1724596..1725270	-	675	WP_010928712.1	response regulator transcription factor	-
B7O99_RS08065	1725400..1727991	+	2592	WP_065956700.1	autotransporter domain-containing protein	-
B7O99_RS08070	1728005..1728304	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
B7O99_RS08075	1728307..1728618	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7O99_RS08080	1728732..1729355	-	624	WP_003811542.1	glutathione S-transferase family protein	-
B7O99_RS08085	1729474..1730427	-	954	WP_041937234.1	LysR family transcriptional regulator	-
B7O99_RS08090	1730544..1731506	+	963	WP_041937418.1	tripartite tricarboxylate transporter substrate binding protein	-
B7O99_RS08095	1731521..1732915	+	1395	WP_003811536.1	arylsulfatase	-
B7O99_RS08100	1732912..1733466	+	555	Protein_1602	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T75214 WP_003811545.1 NZ_CP020648:c1728618-1728307 [Bordetella parapertussis]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T75214 NZ_CP020648:c1728618-1728307 [Bordetella parapertussis]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAACTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT75214 WP_003811547.1 NZ_CP020648:c1728304-1728005 [Bordetella parapertussis]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT75214 NZ_CP020648:c1728304-1728005 [Bordetella parapertussis]
ATGAAAAAAGACAACACTGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3