Detailed information of TA system TA075171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 122155..122740
• Replicon: plasmid pHWBA8_1
• Accession: NZ_CP020596
• Organism: Acinetobacter baumannii strain HWBA8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: B7L46_RS00665
• Protein ID: WP_000897311.1
• Coordinates: 122420..122740 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7L46_RS00660
• Protein ID: WP_000369784.1
• Coordinates: 122155..122427 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L46_RS00625	118022..118201	+	180	Protein_118	IS6 family transposase	-
B7L46_RS00630	118252..118956	-	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
B7L46_RS00635	119021..119422	+	402	Protein_120	hydroxyacid dehydrogenase	-
B7L46_RS00640	119419..120276	+	858	WP_000609033.1	LysR family transcriptional regulator	-
B7L46_RS00645	120602..120814	-	213	WP_001123161.1	hypothetical protein	-
B7L46_RS00650	120930..121231	-	302	Protein_123	transposase	-
B7L46_RS00655	121322..122122	-	801	WP_000604996.1	DUF2971 domain-containing protein	-
B7L46_RS00660	122155..122427	-	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
B7L46_RS00665	122420..122740	-	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7L46_RS00670	123419..124351	-	933	WP_000743272.1	IS5-like element IS17 family transposase	-
B7L46_RS00675	124405..124842	+	438	Protein_128	IS3 family transposase	-
B7L46_RS00680	125100..126332	+	1233	WP_001255535.1	ATP-binding protein	-
B7L46_RS00685	126604..126933	-	330	WP_000506885.1	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	armA / msr(E) / mph(E) / tet(B) / sul2 / aac(6')-Ian / aac(3)-IIa / ARR-3 / cmlA1 / qacE / sul1 / blaPER-7	-	1..195838	195838
	-	inside	IScluster/Tn	aac(6')-Ian / aac(3)-IIa	-	111925..124842	12917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T75171 WP_000897311.1 NZ_CP020596:c122740-122420 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T75171 NZ_CP020596:c122740-122420 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT75171 WP_000369784.1 NZ_CP020596:c122427-122155 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT75171 NZ_CP020596:c122427-122155 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50