Detailed information of TA system TA075169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 60874..61532
• Replicon: plasmid pUSA15_1
• Accession: NZ_CP020594
• Organism: Acinetobacter baumannii strain USA15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B7L41_RS00400
• Protein ID: WP_000312250.1
• Coordinates: 61173..61532 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7L41_RS00395
• Protein ID: WP_001096429.1
• Coordinates: 60874..61173 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L41_RS00350	55898..57178	+	1281	WP_001093570.1	hypothetical protein	-
B7L41_RS00355	57283..57540	+	258	WP_000834290.1	hypothetical protein	-
B7L41_RS00360	57545..58117	+	573	WP_000443897.1	hypothetical protein	-
B7L41_RS00365	58093..58272	+	180	WP_000387630.1	hypothetical protein	-
B7L41_RS00370	58289..58918	+	630	WP_000701003.1	hypothetical protein	-
B7L41_RS00375	58958..59176	+	219	WP_001043201.1	hypothetical protein	-
B7L41_RS00380	59196..59750	+	555	WP_000790084.1	hypothetical protein	-
B7L41_RS00385	59800..60336	+	537	WP_000731978.1	hypothetical protein	-
B7L41_RS00395	60874..61173	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
B7L41_RS00400	61173..61532	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7L41_RS00405	61733..62299	+	567	WP_000710385.1	hypothetical protein	-
B7L41_RS00410	62348..62530	+	183	WP_000373385.1	hypothetical protein	-
B7L41_RS00415	62597..63295	+	699	WP_000873188.1	hypothetical protein	-
B7L41_RS00420	63434..63817	+	384	WP_000654348.1	hypothetical protein	-
B7L41_RS00425	63884..64642	+	759	WP_001053127.1	hypothetical protein	-
B7L41_RS00435	65697..66011	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / blaOXA-23	-	1..98301	98301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T75169 WP_000312250.1 NZ_CP020594:c61532-61173 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T75169 NZ_CP020594:c61532-61173 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT75169 WP_001096429.1 NZ_CP020594:c61173-60874 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT75169 NZ_CP020594:c61173-60874 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA