Detailed information of TA system TA075168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 20097..20755
• Replicon: plasmid pUSA2_1
• Accession: NZ_CP020593
• Organism: Acinetobacter baumannii strain USA2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B7L39_RS18790
• Protein ID: WP_000312250.1
• Coordinates: 20396..20755 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7L39_RS18785
• Protein ID: WP_001096429.1
• Coordinates: 20097..20396 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L39_RS18740	15264..15893	+	630	WP_002081923.1	hypothetical protein	-
B7L39_RS18745	15933..16442	+	510	WP_001043199.1	hypothetical protein	-
B7L39_RS18750	16524..17036	+	513	WP_000840065.1	hypothetical protein	-
B7L39_RS18755	17080..17421	+	342	WP_002001471.1	hypothetical protein	-
B7L39_RS18760	17458..18186	+	729	WP_001022819.1	hypothetical protein	-
B7L39_RS19355	18421..18974	+	554	Protein_28	hypothetical protein	-
B7L39_RS18775	19024..19560	+	537	WP_060853649.1	hypothetical protein	-
B7L39_RS18785	20097..20396	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
B7L39_RS18790	20396..20755	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7L39_RS18795	20956..21522	+	567	WP_031948745.1	hypothetical protein	-
B7L39_RS18800	21571..21753	+	183	WP_000373384.1	hypothetical protein	-
B7L39_RS18805	21820..22518	+	699	WP_000873188.1	hypothetical protein	-
B7L39_RS18810	22657..23040	+	384	WP_000654347.1	hypothetical protein	-
B7L39_RS18815	23107..23865	+	759	WP_060853650.1	hypothetical protein	-
B7L39_RS18820	23917..24252	+	336	WP_000654055.1	hypothetical protein	-
B7L39_RS18830	25117..25431	+	315	WP_000708713.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74241	74241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T75168 WP_000312250.1 NZ_CP020593:c20755-20396 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T75168 NZ_CP020593:c20755-20396 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT75168 WP_001096429.1 NZ_CP020593:c20396-20097 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT75168 NZ_CP020593:c20396-20097 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA