Detailed information of TA system TA075136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5088149..5088744
• Replicon: chromosome
• Accession: NZ_CP020560
• Organism: Pseudomonas aeruginosa strain CR1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: B7D75_RS24090
• Protein ID: WP_003113526.1
• Coordinates: 5088466..5088744 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7D75_RS24085
• Protein ID: WP_034079919.1
• Coordinates: 5088149..5088454 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D75_RS24060	5083175..5084275	+	1101	WP_003151685.1	redox-regulated ATPase YchF	-
B7D75_RS24065	5084347..5085396	-	1050	WP_079741366.1	lipase secretion chaperone	-
B7D75_RS24070	5085477..5086406	-	930	WP_012077421.1	triacylglycerol lipase	-
B7D75_RS24075	5086676..5087785	+	1110	WP_170870656.1	AraC family transcriptional regulator	-
B7D75_RS24085	5088149..5088454	-	306	WP_034079919.1	HigA family addiction module antidote protein	Antitoxin
B7D75_RS24090	5088466..5088744	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7D75_RS24095	5088797..5089789	-	993	WP_107114490.1	tyrosine-type recombinase/integrase	-
B7D75_RS24100	5089786..5091078	-	1293	WP_107114491.1	hypothetical protein	-
B7D75_RS24105	5091308..5092582	-	1275	WP_058174560.1	zonular occludens toxin family protein	-
B7D75_RS24110	5092586..5092942	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5050788..5106452	55664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T75136 WP_003113526.1 NZ_CP020560:c5088744-5088466 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T75136 NZ_CP020560:c5088744-5088466 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGATGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCCATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCTGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACAGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11170.58 Da
• Isoelectric Point: 4.4462

>AT75136 WP_034079919.1 NZ_CP020560:c5088454-5088149 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLTELDISPAALARALNVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT75136 NZ_CP020560:c5088454-5088149 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGACGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGTTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3