Detailed information of TA system TA075131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1183761..1184383
• Replicon: chromosome
• Accession: NZ_CP020560
• Organism: Pseudomonas aeruginosa strain CR1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B7D75_RS05710
• Protein ID: WP_023910260.1
• Coordinates: 1183761..1184075 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7D75_RS05715
• Protein ID: WP_023910258.1
• Coordinates: 1184078..1184383 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D75_RS05680	1179425..1180426	+	1002	WP_079753611.1	site-specific integrase	-
B7D75_RS05685	1180495..1180806	+	312	WP_031631670.1	outer membrane protein assembly factor BamE	-
B7D75_RS05690	1180938..1181588	+	651	WP_058172679.1	BRCT domain-containing protein	-
B7D75_RS05695	1181686..1182159	+	474	WP_124103543.1	hypothetical protein	-
B7D75_RS05700	1182447..1183190	+	744	WP_058172667.1	DUF3800 domain-containing protein	-
B7D75_RS05710	1183761..1184075	+	315	WP_023910260.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7D75_RS05715	1184078..1184383	+	306	WP_023910258.1	helix-turn-helix domain-containing protein	Antitoxin
B7D75_RS05720	1184444..1184743	-	300	WP_058172668.1	hypothetical protein	-
B7D75_RS05725	1184844..1185056	-	213	WP_033967225.1	hypothetical protein	-
B7D75_RS05730	1185053..1186933	-	1881	WP_107113824.1	DNA cytosine methyltransferase	-
B7D75_RS29520	1186930..1187082	-	153	WP_169433150.1	hypothetical protein	-
B7D75_RS05740	1187091..1187825	-	735	WP_033971084.1	hypothetical protein	-
B7D75_RS05745	1187892..1188701	-	810	WP_028680747.1	DUF2303 family protein	-
B7D75_RS05750	1188747..1189094	-	348	WP_031632202.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1173875..1220846	46971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11776.54 Da
• Isoelectric Point: 9.4385

>T75131 WP_023910260.1 NZ_CP020560:1183761-1184075 [Pseudomonas aeruginosa]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGVVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLAY
DKASQEDLTADQKKMLRQIVENWR

Nucleotide

• Length: 315 bp

>T75131 NZ_CP020560:1183761-1184075 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAAGA
AGACCTAACGCTCCACCCGGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGCCTAT
GACAAAGCATCGCAGGAAGATCTGACTGCCGATCAGAAAAAGATGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11316.84 Da
• Isoelectric Point: 7.2077

>AT75131 WP_023910258.1 NZ_CP020560:1184078-1184383 [Pseudomonas aeruginosa]
MDKELFAELVESVTQMDEIVRGERQPSREFHVDAVKVKEIRRATGLSQAKFARKIDVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRES

Nucleotide

• Length: 306 bp

>AT75131 NZ_CP020560:1184078-1184383 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAGGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCCCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCCCTGAATGGTGGCCCGCGAGAAAGCTAA