Detailed information of TA system TA075119

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80673..80937
• Replicon: plasmid pZJ3920-2
• Accession: NZ_CP020547
• Organism: Escherichia coli strain ZJ3920

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: B6V57_RS27540
• Protein ID: WP_001303307.1
• Coordinates: 80673..80825 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	80875..80937 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6V57_RS27500	76187..76396	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
B6V57_RS28745	76605..76781	+	177	WP_001054900.1	hypothetical protein	-
-	77267..77318	+	52	NuclAT_2	-	-
-	77267..77318	+	52	NuclAT_2	-	-
-	77267..77318	+	52	NuclAT_2	-	-
-	77267..77318	+	52	NuclAT_2	-	-
B6V57_RS27520	77735..78490	-	756	WP_001282653.1	IS21-like element ISSso4 family helper ATPase IstB	-
B6V57_RS27525	78507..80042	-	1536	WP_023181050.1	IS21 family transposase	-
B6V57_RS27535	80350..80601	+	252	WP_148123976.1	hypothetical protein	-
B6V57_RS27540	80673..80825	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	80875..80937	+	63	NuclAT_0	-	Antitoxin
-	80875..80937	+	63	NuclAT_0	-	Antitoxin
-	80875..80937	+	63	NuclAT_0	-	Antitoxin
-	80875..80937	+	63	NuclAT_0	-	Antitoxin
B6V57_RS27545	81140..82231	+	1092	WP_000426061.1	hypothetical protein	-
-	82298..82358	+	61	NuclAT_1	-	-
-	82298..82358	+	61	NuclAT_1	-	-
-	82298..82358	+	61	NuclAT_1	-	-
-	82298..82358	+	61	NuclAT_1	-	-
B6V57_RS27550	82538..83746	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
B6V57_RS27555	83765..84835	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / mph(A) / ant(3'')-Ia / blaCTX-M-123	-	1..127809	127809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T75119 WP_001303307.1 NZ_CP020547:c80825-80673 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T75119 NZ_CP020547:c80825-80673 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT75119 NZ_CP020547:80875-80937 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin