Detailed information of TA system TA075109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4305687..4306289
• Replicon: chromosome
• Accession: NZ_CP020545
• Organism: Escherichia coli strain ZJ3920

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: B6V57_RS22400
• Protein ID: WP_000897302.1
• Coordinates: 4305687..4305998 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B6V57_RS22405
• Protein ID: WP_000356397.1
• Coordinates: 4305999..4306289 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6V57_RS22375	4301601..4302200	+	600	WP_001314324.1	glucose-1-phosphatase	-
B6V57_RS22380	4302194..4303066	+	873	WP_000920754.1	virulence factor BrkB family protein	-
B6V57_RS22385	4303063..4303500	+	438	WP_148123957.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B6V57_RS22390	4303545..4304486	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B6V57_RS22395	4304550..4305458	-	909	WP_001331553.1	alpha/beta hydrolase	-
B6V57_RS22400	4305687..4305998	+	312	WP_000897302.1	hypothetical protein	Toxin
B6V57_RS22405	4305999..4306289	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B6V57_RS22410	4306374..4306586	+	213	WP_000197774.1	hypothetical protein	-
B6V57_RS22415	4306648..4306926	+	279	WP_001296612.1	hypothetical protein	-
B6V57_RS22425	4307322..4307540	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
B6V57_RS22430	4307756..4308685	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
B6V57_RS22435	4308682..4309317	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B6V57_RS22440	4309314..4310216	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T75109 WP_000897302.1 NZ_CP020545:4305687-4305998 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T75109 NZ_CP020545:4305687-4305998 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT75109 WP_000356397.1 NZ_CP020545:4305999-4306289 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT75109 NZ_CP020545:4305999-4306289 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416