Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 525776..526455 Replicon chromosome
Accession NZ_CP020545
Organism Escherichia coli strain ZJ3920

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag B6V57_RS02615 Protein ID WP_000057524.1
Coordinates 525776..526078 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag B6V57_RS02620 Protein ID WP_000806442.1
Coordinates 526114..526455 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6V57_RS02585 521152..522804 + 1653 WP_080282699.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
B6V57_RS02590 522842..523345 - 504 WP_000667000.1 hypothetical protein -
B6V57_RS02595 523342..524142 - 801 WP_021561208.1 hypothetical protein -
B6V57_RS02600 524166..524645 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
B6V57_RS02610 524849..525643 - 795 WP_000365177.1 TraB/GumN family protein -
B6V57_RS02615 525776..526078 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B6V57_RS02620 526114..526455 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
B6V57_RS02625 526513..529017 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
B6V57_RS02630 529279..530211 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 522842..535056 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T75085 WP_000057524.1 NZ_CP020545:525776-526078 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T75085 NZ_CP020545:525776-526078 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT75085 WP_000806442.1 NZ_CP020545:526114-526455 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT75085 NZ_CP020545:526114-526455 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References