Detailed information of TA system TA075083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 283983..284677
• Replicon: chromosome
• Accession: NZ_CP020545
• Organism: Escherichia coli strain ZJ3920

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: B6V57_RS01365
• Protein ID: WP_001263500.1
• Coordinates: 284279..284677 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: B6V57_RS01360
• Protein ID: WP_000554758.1
• Coordinates: 283983..284276 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6V57_RS01340	279519..280016	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
B6V57_RS28300	280099..280257	-	159	WP_014639450.1	hypothetical protein	-
B6V57_RS01345	280336..282048	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
B6V57_RS01350	282020..282805	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
B6V57_RS01355	282876..283931	+	1056	WP_001226168.1	DNA polymerase IV	-
B6V57_RS01360	283983..284276	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
B6V57_RS01365	284279..284677	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
B6V57_RS01370	284687..285139	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
B6V57_RS01375	285329..286468	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
B6V57_RS01380	286465..287079	+	615	WP_000602123.1	peptide chain release factor H	-
B6V57_RS01385	287136..288593	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
B6V57_RS01390	288854..289312	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	264458..285139	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T75083 WP_001263500.1 NZ_CP020545:284279-284677 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T75083 NZ_CP020545:284279-284677 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT75083 WP_000554758.1 NZ_CP020545:283983-284276 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT75083 NZ_CP020545:283983-284276 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3