Detailed information of TA system TA075059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 748532..749225
• Replicon: chromosome
• Accession: NZ_CP020543
• Organism: Escherichia coli C

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: B6N50_RS03845
• Protein ID: WP_000415584.1
• Coordinates: 748532..748828 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: B6N50_RS03850
• Protein ID: WP_000650107.1
• Coordinates: 748830..749225 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6N50_RS03810 (B6N50_03810)	743620..743934	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
B6N50_RS03815 (B6N50_03815)	743965..744546	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
B6N50_RS03820 (B6N50_03820)	744865..745197	+	333	WP_000917684.1	DUF2645 family protein	-
B6N50_RS03825 (B6N50_03825)	745243..746592	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
B6N50_RS03830 (B6N50_03830)	746589..747248	-	660	WP_081508774.1	two-component system response regulator QseB	-
B6N50_RS03835 (B6N50_03835)	747400..747792	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
B6N50_RS03840 (B6N50_03840)	747845..748327	+	483	WP_000183505.1	transcriptional regulator	-
B6N50_RS03845 (B6N50_03845)	748532..748828	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
B6N50_RS03850 (B6N50_03850)	748830..749225	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
B6N50_RS03855 (B6N50_03855)	749358..750965	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
B6N50_RS03860 (B6N50_03860)	751103..753361	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T75059 WP_000415584.1 NZ_CP020543:748532-748828 [Escherichia coli C]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T75059 NZ_CP020543:748532-748828 [Escherichia coli C]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT75059 WP_000650107.1 NZ_CP020543:748830-749225 [Escherichia coli C]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT75059 NZ_CP020543:748830-749225 [Escherichia coli C]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65