Detailed information of TA system TA075054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 28607..29187
• Replicon: plasmid pSHV1
• Accession: NZ_CP020540
• Organism: Sphingobium herbicidovorans strain MH

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A086PBV2
• Locus tag: B6S01_RS19240
• Protein ID: WP_037463947.1
• Coordinates: 28607..28888 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: B6S01_RS19245
• Protein ID: WP_037463943.1
• Coordinates: 28900..29187 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S01_RS19215	24272..24763	-	492	WP_037463955.1	hypothetical protein	-
B6S01_RS19225	25560..26333	-	774	WP_051908154.1	Crp/Fnr family transcriptional regulator	-
B6S01_RS19230	27118..27840	+	723	WP_037463951.1	acetoacetyl-CoA reductase	-
B6S01_RS19235	27948..28235	-	288	WP_037463949.1	N-acetyltransferase	-
B6S01_RS19240	28607..28888	+	282	WP_037463947.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B6S01_RS19245	28900..29187	+	288	WP_037463943.1	HigA family addiction module antidote protein	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..312167	312167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10455.92 Da
• Isoelectric Point: 5.8327

>T75054 WP_037463947.1 NZ_CP020540:28607-28888 [Sphingobium herbicidovorans]
MIQSVKGKLVEQVSVGKPGKGFPNDIFRAAVRKLAMLEAAVNLDDLRSPPGNRLEALSDDRAGQYSIRINDQWRICFVWT
DKGPEDVEIVDYH

Nucleotide

• Length: 282 bp

>T75054 NZ_CP020540:28607-28888 [Sphingobium herbicidovorans]
ATGATACAGAGCGTCAAAGGGAAGTTGGTTGAACAGGTGTCGGTGGGCAAGCCGGGCAAAGGCTTCCCGAACGACATCTT
CCGCGCTGCTGTGCGGAAGCTGGCTATGCTTGAGGCGGCGGTGAATCTGGACGACTTGCGGTCGCCTCCGGGGAACAGGC
TGGAAGCGCTGTCCGATGACCGTGCGGGCCAGTATTCGATCCGGATTAACGACCAATGGCGTATCTGTTTCGTCTGGACC
GACAAAGGCCCGGAAGACGTAGAGATCGTGGATTATCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10727.46 Da
• Isoelectric Point: 6.6402

>AT75054 WP_037463943.1 NZ_CP020540:28900-29187 [Sphingobium herbicidovorans]
MTMLKTPVHPGEILKHEFLEEMGISAGKLAKHIHVPRTRIERLCDERTSVTPDTAKRLSKALGTTPEFWLNLQTHYDLLV
DQAADIEEIKPLLAA

Nucleotide

• Length: 288 bp

>AT75054 NZ_CP020540:28900-29187 [Sphingobium herbicidovorans]
ATGACCATGTTGAAAACCCCGGTCCATCCCGGCGAAATCCTCAAGCATGAGTTCCTTGAGGAGATGGGCATTTCCGCTGG
CAAGCTGGCGAAGCATATCCATGTCCCCCGCACCCGCATCGAGCGGCTGTGCGATGAGAGGACTTCGGTCACGCCGGACA
CGGCCAAGCGTTTGTCCAAGGCACTGGGTACGACGCCGGAGTTTTGGCTCAACCTCCAGACACATTATGATTTGCTGGTG
GATCAGGCCGCCGATATCGAAGAAATTAAGCCGCTGTTGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A086PBV2