Detailed information of TA system TA075008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 101023..101680
• Replicon: plasmid unnamed1
• Accession: NZ_CP020524
• Organism: Escherichia coli strain 190

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: A9X72_RS27580
• Protein ID: WP_000270043.1
• Coordinates: 101330..101680 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9X72_RS27575
• Protein ID: WP_000124640.1
• Coordinates: 101023..101325 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X72_RS27525	96657..97085	+	429	WP_000591076.1	hypothetical protein	-
A9X72_RS27530	97143..97502	+	360	WP_000422769.1	hypothetical protein	-
A9X72_RS27535	97502..97948	+	447	WP_000919343.1	hypothetical protein	-
A9X72_RS27540	97945..98463	+	519	WP_000210757.1	nitrite reductase	-
A9X72_RS27545	98463..98693	+	231	WP_000972665.1	hypothetical protein	-
A9X72_RS27550	98680..99537	+	858	WP_001167036.1	hypothetical protein	-
A9X72_RS28525	99449..99754	+	306	WP_171820680.1	hypothetical protein	-
A9X72_RS27560	99757..100284	+	528	WP_004201083.1	thermonuclease family protein	-
A9X72_RS27565	100342..100614	+	273	WP_001043046.1	HU family DNA-binding protein	-
A9X72_RS27570	100703..100996	+	294	WP_001239998.1	hypothetical protein	-
A9X72_RS27575	101023..101325	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
A9X72_RS27580	101330..101680	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9X72_RS27585	101843..102391	+	549	WP_001061573.1	hypothetical protein	-
A9X72_RS27595	102732..102926	+	195	WP_000343597.1	hypothetical protein	-
A9X72_RS27600	102937..103308	+	372	WP_000516918.1	hypothetical protein	-
A9X72_RS27605	103301..103771	+	471	WP_001281821.1	hypothetical protein	-
A9X72_RS27610	103786..104121	-	336	WP_000683477.1	hypothetical protein	-
A9X72_RS27615	104218..104706	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
A9X72_RS27620	104709..105206	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / cmlA1 / qacE / sul1 / rmtC / aph(3')-Ia / aph(3')-VI / blaNDM-1 / blaCMY-6	htpB	1..177196	177196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T75008 WP_000270043.1 NZ_CP020524:c101680-101330 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T75008 NZ_CP020524:c101680-101330 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT75008 WP_000124640.1 NZ_CP020524:c101325-101023 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT75008 NZ_CP020524:c101325-101023 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9