Detailed information of TA system TA074982

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103641..103881
• Replicon: plasmid unnamed1
• Accession: NZ_CP020519
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A9P13_RS28505
• Protein ID: WP_001312861.1
• Coordinates: 103641..103799 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	103843..103881 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS28480	99149..99970	-	822	WP_001234469.1	DUF945 domain-containing protein	-
A9P13_RS28485	100089..100376	-	288	WP_000107535.1	hypothetical protein	-
A9P13_RS29035	100401..100607	-	207	WP_024190427.1	hypothetical protein	-
A9P13_RS28500	101579..103246	+	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
A9P13_RS28505	103641..103799	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	103843..103881	-	39	NuclAT_1	-	Antitoxin
-	103843..103881	-	39	NuclAT_1	-	Antitoxin
-	103843..103881	-	39	NuclAT_1	-	Antitoxin
-	103843..103881	-	39	NuclAT_1	-	Antitoxin
-	105321..105423	-	103	NuclAT_0	-	-
-	105321..105423	-	103	NuclAT_0	-	-
-	105321..105423	-	103	NuclAT_0	-	-
-	105321..105423	-	103	NuclAT_0	-	-
A9P13_RS28515	105435..106154	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
A9P13_RS28520	106151..106585	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
A9P13_RS28525	106640..108598	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId	-	1..120667	120667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T74982 WP_001312861.1 NZ_CP020519:c103799-103641 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T74982 NZ_CP020519:c103799-103641 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT74982 NZ_CP020519:c103881-103843 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin