Detailed information of TA system TA074978

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75256..75495
• Replicon: plasmid unnamed1
• Accession: NZ_CP020519
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: A9P13_RS28320
• Protein ID: WP_023144756.1
• Coordinates: 75256..75390 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75435..75495 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS28290	70494..70568	-	75	WP_031943482.1	RepA leader peptide Tap	-
A9P13_RS29025	70565..70699	-	135	Protein_82	protein CopA/IncA	-
A9P13_RS28300	70825..72038	+	1214	WP_162829202.1	IS3 family transposase	-
A9P13_RS28305	72243..73784	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
A9P13_RS28310	73799..74545	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
A9P13_RS28315	74705..74959	-	255	WP_000083850.1	replication regulatory protein RepA	-
A9P13_RS28320	75256..75390	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	75435..75495	+	61	NuclAT_2	-	Antitoxin
-	75435..75495	+	61	NuclAT_2	-	Antitoxin
-	75435..75495	+	61	NuclAT_2	-	Antitoxin
-	75435..75495	+	61	NuclAT_2	-	Antitoxin
A9P13_RS29030	75462..75748	-	287	Protein_88	DUF2726 domain-containing protein	-
A9P13_RS28335	76261..76473	-	213	WP_013023861.1	hypothetical protein	-
A9P13_RS28340	76604..77164	-	561	WP_000139341.1	fertility inhibition protein FinO	-
A9P13_RS28345	77219..77965	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
A9P13_RS28350	77985..80441	-	2457	Protein_92	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId	-	1..120667	120667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T74978 WP_023144756.1 NZ_CP020519:c75390-75256 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T74978 NZ_CP020519:c75390-75256 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT74978 NZ_CP020519:75435-75495 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin