Detailed information of TA system TA074974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 15449..16095
• Replicon: plasmid unnamed2
• Accession: NZ_CP020518
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A9P13_RS27620
• Protein ID: WP_000269912.1
• Coordinates: 15748..16095 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: A9P13_RS27615
• Protein ID: WP_001259436.1
• Coordinates: 15449..15748 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS27585	11366..11557	+	192	WP_000183351.1	hypothetical protein	-
A9P13_RS27590	11554..12696	+	1143	WP_032154032.1	ORF6N domain-containing protein	-
A9P13_RS27595	12731..13366	-	636	WP_032154031.1	hypothetical protein	-
A9P13_RS27600	14023..14274	-	252	WP_039052397.1	helix-turn-helix domain-containing protein	-
A9P13_RS27605	14376..14801	+	426	WP_016240784.1	hypothetical protein	-
A9P13_RS27610	14905..15285	-	381	WP_001523084.1	hypothetical protein	-
A9P13_RS27615	15449..15748	-	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
A9P13_RS27620	15748..16095	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9P13_RS27625	16340..16603	-	264	WP_000424604.1	hypothetical protein	-
A9P13_RS27630	16627..16914	-	288	WP_000356589.1	hypothetical protein	-
A9P13_RS27635	17558..18394	-	837	WP_001139597.1	hypothetical protein	-
A9P13_RS27640	18746..19273	-	528	WP_050015645.1	tail fiber assembly protein	-
A9P13_RS27645	19275..19877	-	603	Protein_35	phage tail protein	-
A9P13_RS27650	20325..20603	-	279	WP_050015643.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48175	48175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T74974 WP_000269912.1 NZ_CP020518:c16095-15748 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T74974 NZ_CP020518:c16095-15748 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATCAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAACGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT74974 WP_001259436.1 NZ_CP020518:c15748-15449 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT74974 NZ_CP020518:c15748-15449 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V3UZT0