Detailed information of TA system TA074970

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100053..100479
• Replicon: plasmid unnamed3
• Accession: NZ_CP020517
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A9P13_RS27420
• Protein ID: WP_001312861.1
• Coordinates: 100053..100211 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100255..100479 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS27370	95076..95303	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
A9P13_RS27375	95440..96111	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
A9P13_RS27380	96305..96688	-	384	WP_001354030.1	relaxosome protein TraM	-
A9P13_RS27390	97011..97613	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
A9P13_RS27395	97910..98731	-	822	WP_001234445.1	DUF945 domain-containing protein	-
A9P13_RS27400	98842..99138	-	297	WP_001272251.1	hypothetical protein	-
A9P13_RS29005	99438..99815	+	378	Protein_116	hypothetical protein	-
A9P13_RS27420	100053..100211	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	100255..100479	-	225	NuclAT_0	-	Antitoxin
-	100255..100479	-	225	NuclAT_0	-	Antitoxin
-	100255..100479	-	225	NuclAT_0	-	Antitoxin
-	100255..100479	-	225	NuclAT_0	-	Antitoxin
A9P13_RS28840	100291..100470	+	180	WP_001309233.1	hypothetical protein	-
A9P13_RS27425	100491..101210	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
A9P13_RS27430	101207..101641	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
A9P13_RS27435	101710..103733	-	2024	Protein_121	ParB/RepB/Spo0J family partition protein	-
A9P13_RS27440	103794..104027	-	234	WP_000006003.1	DUF905 family protein	-
A9P13_RS27445	104085..104570	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / erm(B) / qnrS1	-	1..109864	109864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T74970 WP_001312861.1 NZ_CP020517:c100211-100053 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T74970 NZ_CP020517:c100211-100053 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT74970 NZ_CP020517:c100479-100255 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin