Detailed information of TA system TA074962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3993303..3993997
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: A9P13_RS20740
• Protein ID: WP_001521903.1
• Coordinates: 3993303..3993701 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: A9P13_RS20745
• Protein ID: WP_000554758.1
• Coordinates: 3993704..3993997 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS20710	3988473..3989717	-	1245	WP_000189541.1	esterase FrsA	-
-	3989133..3989213	-	81	NuclAT_11	-	-
-	3989133..3989213	-	81	NuclAT_11	-	-
-	3989133..3989213	-	81	NuclAT_11	-	-
-	3989133..3989213	-	81	NuclAT_11	-	-
A9P13_RS20715	3989809..3990267	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A9P13_RS20720	3990528..3991985	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A9P13_RS20725	3992042..3992394	-	353	Protein_3794	peptide chain release factor H	-
A9P13_RS20730	3992390..3992596	-	207	Protein_3795	RtcB family protein	-
A9P13_RS20735	3992841..3993293	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
A9P13_RS20740	3993303..3993701	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A9P13_RS20745	3993704..3993997	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A9P13_RS20750	3994049..3995104	-	1056	WP_001226177.1	DNA polymerase IV	-
A9P13_RS20755	3995175..3995960	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A9P13_RS20760	3995932..3997644	+	1713	Protein_3801	flagellar biosynthesis protein FlhA	-
A9P13_RS20765	3997723..3998076	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
A9P13_RS20770	3998133..3998882	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3983240..3993997	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T74962 WP_001521903.1 NZ_CP020516:c3993701-3993303 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T74962 NZ_CP020516:c3993701-3993303 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT74962 WP_000554758.1 NZ_CP020516:c3993997-3993704 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT74962 NZ_CP020516:c3993997-3993704 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3