Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3169277..3169779 Replicon chromosome
Accession NZ_CP020516
Organism Escherichia coli strain 222

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A9P13_RS16645 Protein ID WP_000767829.1
Coordinates 3169277..3169531 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A9P13_RS16650 Protein ID WP_001259255.1
Coordinates 3169528..3169779 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9P13_RS16615 3164292..3164519 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A9P13_RS16620 3164533..3165591 - 1059 WP_000492324.1 transport protein YeeE -
A9P13_RS16625 3165770..3167128 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A9P13_RS16630 3167118..3167180 - 63 WP_010723108.1 membrane protein YoeI -
A9P13_RS16635 3167395..3168324 - 930 WP_023154044.1 LysR family transcriptional regulator -
A9P13_RS16640 3168370..3169194 - 825 WP_000754758.1 SDR family oxidoreductase -
A9P13_RS16645 3169277..3169531 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A9P13_RS16650 3169528..3169779 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A9P13_RS16655 3170062..3170112 + 51 WP_001364200.1 his operon leader peptide -
A9P13_RS16665 3170258..3171157 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
A9P13_RS16670 3171163..3172467 + 1305 WP_021523144.1 histidinol dehydrogenase -
A9P13_RS16675 3172464..3173534 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
A9P13_RS16680 3173534..3174601 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T74958 WP_000767829.1 NZ_CP020516:c3169531-3169277 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T74958 NZ_CP020516:c3169531-3169277 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT74958 WP_001259255.1 NZ_CP020516:c3169779-3169528 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT74958 NZ_CP020516:c3169779-3169528 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References