Detailed information of TA system TA074943

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2392075..2392446
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: A9P13_RS12440
• Protein ID: WP_001443846.1
• Coordinates: 2392297..2392446 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2392075..2392253 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS12415	2388030..2389403	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
A9P13_RS12420	2389532..2390467	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
A9P13_RS12425	2390519..2391754	-	1236	WP_000040839.1	site-specific integrase	-
A9P13_RS12430	2391756..2391971	-	216	WP_000079604.1	excisionase XisR	-
A9P13_RS12435	2392071..2392259	-	189	WP_001302840.1	DUF1187 family protein	-
-	2392075..2392253	+	179	NuclAT_0	-	Antitoxin
-	2392075..2392253	+	179	NuclAT_0	-	Antitoxin
-	2392075..2392253	+	179	NuclAT_0	-	Antitoxin
-	2392075..2392253	+	179	NuclAT_0	-	Antitoxin
A9P13_RS12440	2392297..2392446	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
A9P13_RS12445	2392502..2393311	-	810	WP_000166313.1	recombination protein RecT	-
A9P13_RS12450	2393304..2395904	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
A9P13_RS12455	2396006..2396281	-	276	WP_001344816.1	hypothetical protein	-
A9P13_RS12460	2396356..2396526	-	171	WP_001352098.1	conserved protein, Rac prophage	-
A9P13_RS12465	2396526..2396747	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2338900..2437520	98620

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T74943 WP_001443846.1 NZ_CP020516:c2392446-2392297 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T74943 NZ_CP020516:c2392446-2392297 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT74943 NZ_CP020516:2392075-2392253 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin