Detailed information of TA system TA074927

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53950..54371
• Replicon: plasmid unnamed
• Accession: NZ_CP020515
• Organism: Escherichia coli strain 219

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: A9P12_RS26055
• Protein ID: WP_001302184.1
• Coordinates: 53950..54108 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54173..54371 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P12_RS26020	49014..49229	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
A9P12_RS26025	49365..50012	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
A9P12_RS26030	50203..50586	-	384	WP_001063020.1	relaxosome protein TraM	-
A9P12_RS26035	50956..51528	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
A9P12_RS26040	51824..52645	-	822	WP_001234465.1	DUF945 domain-containing protein	-
A9P12_RS26045	52764..53051	-	288	WP_000107242.1	hypothetical protein	-
A9P12_RS27215	53076..53282	-	207	WP_000275854.1	hypothetical protein	-
A9P12_RS26055	53950..54108	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54173..54371	-	199	NuclAT_0	-	Antitoxin
-	54173..54371	-	199	NuclAT_0	-	Antitoxin
-	54173..54371	-	199	NuclAT_0	-	Antitoxin
-	54173..54371	-	199	NuclAT_0	-	Antitoxin
A9P12_RS26060	54340..54445	-	106	Protein_66	plasmid SOS inhibition protein A	-
A9P12_RS26065	54600..54917	+	318	Protein_67	IS110 family transposase	-
A9P12_RS26070	55206..55457	-	252	Protein_68	iron-containing alcohol dehydrogenase	-
A9P12_RS26075	55542..56165	-	624	WP_000912556.1	potassium channel family protein	-
A9P12_RS26080	56181..56874	-	694	Protein_70	IS1 family transposase	-
A9P12_RS26090	56931..57035	+	105	Protein_71	thiosulfate reductase cytochrome B subunit	-
A9P12_RS26100	57281..58675	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..158757	158757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T74927 WP_001302184.1 NZ_CP020515:c54108-53950 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T74927 NZ_CP020515:c54108-53950 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT74927 NZ_CP020515:c54371-54173 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin