Detailed information of TA system TA074925

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16828..17082
• Replicon: plasmid unnamed
• Accession: NZ_CP020515
• Organism: Escherichia coli strain 219

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: A9P12_RS25825
• Protein ID: WP_015387399.1
• Coordinates: 16828..17034 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	17021..17082 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P12_RS25790	12075..12986	-	912	WP_000440183.1	carbamate kinase	-
A9P12_RS25795	12997..14217	-	1221	WP_000410951.1	arginine deiminase	-
A9P12_RS25800	15110..15592	+	483	Protein_18	VENN motif pre-toxin domain-containing protein	-
A9P12_RS25805	15538..15984	-	447	Protein_19	incFII family plasmid replication initiator RepA	-
A9P12_RS25810	15977..16051	-	75	WP_001365705.1	RepA leader peptide Tap	-
A9P12_RS25820	16287..16544	-	258	WP_000083833.1	replication regulatory protein RepA	-
A9P12_RS25825	16828..17034	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	17021..17082	+	62	NuclAT_1	-	Antitoxin
-	17021..17082	+	62	NuclAT_1	-	Antitoxin
-	17021..17082	+	62	NuclAT_1	-	Antitoxin
-	17021..17082	+	62	NuclAT_1	-	Antitoxin
A9P12_RS25835	17221..17403	-	183	WP_000968309.1	hypothetical protein	-
A9P12_RS25845	17504..18206	+	703	Protein_24	IS1 family transposase	-
A9P12_RS25855	18507..18719	-	213	WP_005012601.1	hypothetical protein	-
A9P12_RS25860	18852..19412	-	561	WP_000139363.1	fertility inhibition protein FinO	-
A9P12_RS25865	19467..20213	-	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..158757	158757
	-	flank	IS/Tn	-	-	17820..18206	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T74925 WP_015387399.1 NZ_CP020515:c17034-16828 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T74925 NZ_CP020515:c17034-16828 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT74925 NZ_CP020515:17021-17082 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin