Detailed information of TA system TA074921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3891394..3892088
• Replicon: chromosome
• Accession: NZ_CP020514
• Organism: Escherichia coli strain 219

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: A9P12_RS20050
• Protein ID: WP_001263489.1
• Coordinates: 3891394..3891792 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: A9P12_RS20055
• Protein ID: WP_000554758.1
• Coordinates: 3891795..3892088 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3886982..3887062	-	81	NuclAT_13	-	-
-	3886982..3887062	-	81	NuclAT_13	-	-
-	3886982..3887062	-	81	NuclAT_13	-	-
-	3886982..3887062	-	81	NuclAT_13	-	-
A9P12_RS20020	3887658..3888116	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A9P12_RS20025	3888377..3889834	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
A9P12_RS20030	3889891..3890412	-	522	Protein_3658	peptide chain release factor H	-
A9P12_RS20035	3890408..3890614	-	207	Protein_3659	RtcB family protein	-
A9P12_RS20045	3890932..3891384	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
A9P12_RS20050	3891394..3891792	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A9P12_RS20055	3891795..3892088	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A9P12_RS20060	3892140..3893195	-	1056	WP_001226164.1	DNA polymerase IV	-
A9P12_RS20065	3893266..3894051	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
A9P12_RS20070	3894023..3895735	+	1713	Protein_3665	flagellar biosynthesis protein FlhA	-
A9P12_RS20075	3895959..3896456	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3891394..3906673	15279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T74921 WP_001263489.1 NZ_CP020514:c3891792-3891394 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T74921 NZ_CP020514:c3891792-3891394 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT74921 WP_000554758.1 NZ_CP020514:c3892088-3891795 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT74921 NZ_CP020514:c3892088-3891795 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3