Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1940722..1941224 Replicon chromosome
Accession NZ_CP020514
Organism Escherichia coli strain 219

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A9P12_RS09925 Protein ID WP_000767829.1
Coordinates 1940970..1941224 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A9P12_RS09920 Protein ID WP_001259255.1
Coordinates 1940722..1940973 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9P12_RS09890 1935899..1936966 - 1068 WP_000080055.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
A9P12_RS09895 1936966..1938036 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
A9P12_RS09900 1938033..1939337 - 1305 WP_001352244.1 histidinol dehydrogenase -
A9P12_RS09905 1939343..1940242 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
A9P12_RS09915 1940388..1940438 - 51 WP_001364200.1 his operon leader peptide -
A9P12_RS09920 1940722..1940973 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A9P12_RS09925 1940970..1941224 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A9P12_RS09930 1941307..1942131 + 825 WP_000754737.1 SDR family oxidoreductase -
A9P12_RS09935 1942177..1943106 + 930 WP_000803366.1 LysR family transcriptional regulator -
A9P12_RS09940 1943321..1943383 + 63 WP_010723108.1 membrane protein YoeI -
A9P12_RS09945 1943373..1944731 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A9P12_RS09950 1944910..1945968 + 1059 WP_000492346.1 transport protein YeeE -
A9P12_RS09955 1945982..1946209 + 228 WP_000985272.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T74906 WP_000767829.1 NZ_CP020514:1940970-1941224 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T74906 NZ_CP020514:1940970-1941224 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT74906 WP_001259255.1 NZ_CP020514:1940722-1940973 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT74906 NZ_CP020514:1940722-1940973 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References