Detailed information of TA system TA074894

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48482..48746
• Replicon: plasmid unnamed2
• Accession: NZ_CP020511
• Organism: Escherichia coli strain 165

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: AWB10_RS27670
• Protein ID: WP_001387489.1
• Coordinates: 48482..48634 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	48686..48746 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWB10_RS27645	43748..45916	+	2169	WP_015508354.1	DotA/TraY family protein	-
AWB10_RS27650	45987..46649	+	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
AWB10_RS27655	46721..46930	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AWB10_RS29120	47322..47498	+	177	WP_001054900.1	hypothetical protein	-
AWB10_RS27660	47563..47859	-	297	WP_011264046.1	hypothetical protein	-
AWB10_RS27665	48159..48410	+	252	WP_001291964.1	hypothetical protein	-
AWB10_RS27670	48482..48634	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	48686..48746	+	61	NuclAT_0	-	Antitoxin
-	48686..48746	+	61	NuclAT_0	-	Antitoxin
-	48686..48746	+	61	NuclAT_0	-	Antitoxin
-	48686..48746	+	61	NuclAT_0	-	Antitoxin
AWB10_RS27675	48949..50040	+	1092	WP_000426061.1	hypothetical protein	-
-	50110..50167	+	58	NuclAT_1	-	-
-	50110..50167	+	58	NuclAT_1	-	-
-	50110..50167	+	58	NuclAT_1	-	-
-	50110..50167	+	58	NuclAT_1	-	-
AWB10_RS27680	50347..51555	+	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
AWB10_RS27685	51574..52644	+	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85008	85008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T74894 WP_001387489.1 NZ_CP020511:c48634-48482 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T74894 NZ_CP020511:c48634-48482 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT74894 NZ_CP020511:48686-48746 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin