Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 65627..65897 Replicon plasmid unnamed1
Accession NZ_CP020510
Organism Escherichia coli strain 165

Toxin (Protein)


Gene name hok Uniprot ID -
Locus tag AWB10_RS27035 Protein ID WP_034169443.1
Coordinates 65739..65897 (+) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 65627..65690 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB10_RS28835 60655..60924 + 270 WP_074015062.1 hypothetical protein -
AWB10_RS29090 61165..61371 + 207 WP_000547971.1 hypothetical protein -
AWB10_RS27010 61397..61936 + 540 WP_009426937.1 single-stranded DNA-binding protein -
AWB10_RS27015 61998..62231 + 234 WP_000006030.1 DUF905 family protein -
AWB10_RS27020 62296..64254 + 1959 WP_072652246.1 ParB/RepB/Spo0J family partition protein -
AWB10_RS27025 64309..64743 + 435 WP_032217500.1 conjugation system SOS inhibitor PsiB -
AWB10_RS27030 64740..65459 + 720 WP_050858785.1 plasmid SOS inhibition protein A -
AWB10_RS28840 65471..65659 - 189 WP_001299721.1 hypothetical protein -
- 65471..65695 + 225 NuclAT_0 - -
- 65471..65695 + 225 NuclAT_0 - -
- 65471..65695 + 225 NuclAT_0 - -
- 65471..65695 + 225 NuclAT_0 - -
- 65627..65690 - 64 - - Antitoxin
AWB10_RS27035 65739..65897 + 159 WP_034169443.1 type I toxin-antitoxin system Hok family toxin Toxin
AWB10_RS29095 66588..66794 + 207 WP_000547971.1 hypothetical protein -
AWB10_RS27055 66819..67106 + 288 WP_000107535.1 hypothetical protein -
AWB10_RS27060 67224..68045 + 822 WP_001234469.1 DUF945 domain-containing protein -
AWB10_RS27065 68342..68989 - 648 WP_000614935.1 transglycosylase SLT domain-containing protein -
AWB10_RS27070 69266..69649 + 384 WP_000124981.1 relaxosome protein TraM -
AWB10_RS27075 69840..70526 + 687 WP_001825184.1 PAS domain-containing protein -
AWB10_RS27080 70620..70847 + 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / rmtB / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) - 1..118182 118182


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-48)


Sequences


Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T74890 WP_034169443.1 NZ_CP020510:65739-65897 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCELRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T74890 NZ_CP020510:65739-65897 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGCTTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 64 bp

>AT74890 NZ_CP020510:c65690-65627 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Download structure file

References