Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1919647..1920149 Replicon chromosome
Accession NZ_CP020509
Organism Escherichia coli strain 165

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AWB10_RS09690 Protein ID WP_000767829.1
Coordinates 1919895..1920149 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AWB10_RS09685 Protein ID WP_001259255.1
Coordinates 1919647..1919898 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB10_RS09655 1914826..1915893 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AWB10_RS09660 1915893..1916963 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
AWB10_RS09665 1916960..1918264 - 1305 WP_001515511.1 histidinol dehydrogenase -
AWB10_RS09670 1918270..1919169 - 900 WP_061093169.1 ATP phosphoribosyltransferase -
AWB10_RS09680 1919315..1919365 - 51 WP_001364200.1 his operon leader peptide -
AWB10_RS09685 1919647..1919898 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AWB10_RS09690 1919895..1920149 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AWB10_RS09695 1920232..1921056 + 825 WP_000754763.1 SDR family oxidoreductase -
AWB10_RS09700 1921102..1922031 + 930 WP_000803351.1 LysR family transcriptional regulator -
AWB10_RS09705 1922246..1922308 + 63 WP_010723108.1 membrane protein YoeI -
AWB10_RS09710 1922298..1923656 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AWB10_RS09715 1923835..1924893 + 1059 WP_023568741.1 transport protein YeeE -
AWB10_RS09720 1924907..1925134 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T74871 WP_000767829.1 NZ_CP020509:1919895-1920149 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T74871 NZ_CP020509:1919895-1920149 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT74871 WP_001259255.1 NZ_CP020509:1919647-1919898 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT74871 NZ_CP020509:1919647-1919898 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References