Detailed information of TA system TA074844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 514951..515604
• Replicon: chromosome
• Accession: NZ_CP020507
• Organism: Serratia marcescens strain BWH-35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AS658_RS02440
• Protein ID: WP_038878324.1
• Coordinates: 514951..515301 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: AS658_RS02445
• Protein ID: WP_019453270.1
• Coordinates: 515305..515604 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS658_RS02405	510089..510499	-	411	WP_048232572.1	GNAT family N-acetyltransferase	-
AS658_RS02410	510529..511899	-	1371	WP_048232574.1	glutamine synthetase family protein	-
AS658_RS02415	512145..512444	+	300	WP_048232576.1	hypothetical protein	-
AS658_RS02425	512869..513265	-	397	Protein_446	hypothetical protein	-
AS658_RS02430	513279..513671	-	393	WP_038878318.1	hypothetical protein	-
AS658_RS02435	513675..514679	-	1005	WP_038878320.1	hypothetical protein	-
AS658_RS02440	514951..515301	+	351	WP_038878324.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AS658_RS02445	515305..515604	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
AS658_RS02450	515612..516511	-	900	WP_038878327.1	LysR family transcriptional regulator	-
AS658_RS02455	516680..517378	+	699	WP_004933280.1	CoA transferase subunit A	-
AS658_RS02460	517390..518043	+	654	WP_038878331.1	CoA transferase subunit B	-
AS658_RS02465	518058..519242	+	1185	WP_038878334.1	acetyl-CoA C-acetyltransferase	-
AS658_RS02470	519261..520167	+	907	Protein_455	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13574.83 Da
• Isoelectric Point: 8.2712

>T74844 WP_038878324.1 NZ_CP020507:514951-515301 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLRGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T74844 NZ_CP020507:514951-515301 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAACAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCAGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAGAGGCTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT74844 WP_019453270.1 NZ_CP020507:515305-515604 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT74844 NZ_CP020507:515305-515604 [Serratia marcescens]
ATGAGCACCCTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGTTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3