Detailed information of TA system TA074835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4224351..4225003
• Replicon: chromosome
• Accession: NZ_CP020503
• Organism: Serratia marcescens strain 95

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM681_RS20435
• Protein ID: WP_038878669.1
• Coordinates: 4224351..4224695 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GZD7
• Locus tag: AM681_RS20440
• Protein ID: WP_038878665.1
• Coordinates: 4224701..4225003 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM681_RS20415	4220507..4220692	-	186	WP_038878678.1	hypothetical protein	-
AM681_RS20420	4220693..4222951	-	2259	WP_038878674.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-
AM681_RS20430	4223172..4224191	+	1020	WP_038878671.1	HTH-type transcriptional regulator GalR	-
AM681_RS20435	4224351..4224695	+	345	WP_038878669.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM681_RS20440	4224701..4225003	+	303	WP_038878665.1	helix-turn-helix transcriptional regulator	Antitoxin
AM681_RS20445	4225031..4226293	-	1263	WP_038878662.1	diaminopimelate decarboxylase	-
AM681_RS20450	4226427..4227350	+	924	WP_038878659.1	LysR family transcriptional regulator	-
AM681_RS20455	4227378..4228286	-	909	WP_038878655.1	LysR family transcriptional regulator	-
AM681_RS20460	4228395..4229279	+	885	WP_033635778.1	MBL fold metallo-hydrolase	-
AM681_RS20465	4229348..4229995	+	648	WP_038878652.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13367.40 Da
• Isoelectric Point: 10.6629

>T74835 WP_038878669.1 NZ_CP020503:4224351-4224695 [Serratia marcescens]
MWQVVTVERFDDWFLALNNAQQTSILAAIFKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDQRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T74835 NZ_CP020503:4224351-4224695 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTCGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTTCAAATTACAGACGTTCGGCCCGCAATTGGCACGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCATTCAACATCGCGGGCGCCCTTTCAGGGTATTTTTCGCCTTTGACCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACCAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11111.04 Da
• Isoelectric Point: 10.3986

>AT74835 WP_038878665.1 NZ_CP020503:4224701-4225003 [Serratia marcescens]
MAKPLSQLIDKLDPAVVATARQKADKEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT74835 NZ_CP020503:4224701-4225003 [Serratia marcescens]
ATGGCCAAACCTTTATCCCAACTGATCGACAAGCTCGATCCCGCCGTCGTTGCCACTGCCAGGCAAAAAGCGGACAAGGA
AATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGCTGGCGGTCTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATCAAACTGTCATCGCTCAAACGCTATATCGAGGCGATG
GGTGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGCCGCATGACGCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GZD7