Detailed information of TA system TA074821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 516099..516752
• Replicon: chromosome
• Accession: NZ_CP020503
• Organism: Serratia marcescens strain 95

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM681_RS02450
• Protein ID: WP_038878324.1
• Coordinates: 516099..516449 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: AM681_RS02455
• Protein ID: WP_019453270.1
• Coordinates: 516453..516752 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM681_RS02415	511237..511647	-	411	WP_048232572.1	GNAT family N-acetyltransferase	-
AM681_RS02420	511677..513047	-	1371	WP_048232574.1	glutamine synthetase family protein	-
AM681_RS02425	513293..513592	+	300	WP_048232576.1	hypothetical protein	-
AM681_RS02435	514017..514413	-	397	Protein_448	hypothetical protein	-
AM681_RS02440	514427..514819	-	393	WP_038878318.1	hypothetical protein	-
AM681_RS02445	514823..515827	-	1005	WP_038878320.1	hypothetical protein	-
AM681_RS02450	516099..516449	+	351	WP_038878324.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM681_RS02455	516453..516752	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
AM681_RS02460	516760..517659	-	900	WP_038878327.1	LysR family transcriptional regulator	-
AM681_RS02465	517828..518526	+	699	WP_004933280.1	CoA transferase subunit A	-
AM681_RS02470	518538..519191	+	654	WP_038878331.1	CoA transferase subunit B	-
AM681_RS02475	519206..520390	+	1185	WP_038878334.1	acetyl-CoA C-acetyltransferase	-
AM681_RS02480	520409..521315	+	907	Protein_457	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13574.83 Da
• Isoelectric Point: 8.2712

>T74821 WP_038878324.1 NZ_CP020503:516099-516449 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLRGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T74821 NZ_CP020503:516099-516449 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAACAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCAGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAGAGGCTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT74821 WP_019453270.1 NZ_CP020503:516453-516752 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT74821 NZ_CP020503:516453-516752 [Serratia marcescens]
ATGAGCACCCTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGTTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3