Detailed information of TA system TA074797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 34526..35172
• Replicon: plasmid unnamed2
• Accession: NZ_CP020497
• Organism: Escherichia coli strain 103

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CPA47_RS26495
• Protein ID: WP_000269912.1
• Coordinates: 34526..34873 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: CPA47_RS26500
• Protein ID: WP_001259436.1
• Coordinates: 34873..35172 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA47_RS26465	30018..30296	+	279	WP_050015643.1	hypothetical protein	-
CPA47_RS26900	30744..31346	+	603	Protein_41	phage tail protein	-
CPA47_RS26475	31348..31875	+	528	WP_050015645.1	tail fiber assembly protein	-
CPA47_RS26480	32227..33063	+	837	WP_001139597.1	hypothetical protein	-
CPA47_RS26485	33707..33994	+	288	WP_000356589.1	hypothetical protein	-
CPA47_RS26490	34018..34281	+	264	WP_000424604.1	hypothetical protein	-
CPA47_RS26495	34526..34873	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPA47_RS26500	34873..35172	+	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
CPA47_RS26505	35336..35716	+	381	WP_001523084.1	hypothetical protein	-
CPA47_RS26510	35820..36245	-	426	WP_016240784.1	hypothetical protein	-
CPA47_RS26515	36347..36598	+	252	WP_039052397.1	helix-turn-helix domain-containing protein	-
CPA47_RS26520	37254..37889	+	636	WP_032154031.1	hypothetical protein	-
CPA47_RS26525	37924..39066	-	1143	WP_032154032.1	ORF6N domain-containing protein	-
CPA47_RS26530	39063..39254	-	192	WP_000183351.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48172	48172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T74797 WP_000269912.1 NZ_CP020497:34526-34873 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T74797 NZ_CP020497:34526-34873 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATCAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAACGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT74797 WP_001259436.1 NZ_CP020497:34873-35172 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT74797 NZ_CP020497:34873-35172 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V3UZT0